PCR Mutation Detection Protocols
1st Edition
Published on 10. November 2010
Book
Paperback/Softback
224 pages
978-1-61737-100-4 (ISBN)
Description
1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
Reviews / Votes
"The book provides access to the highly productive and readily reproducible PCR-based mutation detection methods, that are behind many of today's major scientific and medical advances." - Clinical Laboratory InternationalMore details
Series
Edition
1., st Edition. Softcover version of original hardcover edition 2002
Language
English
Place of publication
Totowa, NJ
United States
Target group
College/higher education
Professional and scholarly
Research
Illustrations
21
21 s/w Abbildungen
21 black & white illustrations
Dimensions
Height: 22.9 cm
Width: 15.2 cm
Weight
328 gr
ISBN-13
978-1-61737-100-4 (9781617371004)
Schweitzer Classification
Other editions
Additional editions
Bimal D. M. Theophilus | Ralph Rapley
PCR Mutation Detection Protocols
Book
04/2002
Humana Press Inc.
€85.59
Article exhausted; check for reprint
Content
Agarose and Polyacrylamide Gel Electrophoresis
Andrea M. Guilliatt
Internal Labeling of DNA Probes
Ralph Rapley and Bimal D. M. Theophilus
End-Labeling of DNA Probes
Adrian J. Harwood
Southern Blotting of Agarose Gels by Capillary Transfer
Ralph Rapley and Ian J. Williams
Restriction Fragment Length Polymorphism
Mohammad S. Enayat
PCR: Principles, Procedures, and Parameters
Nicola Louise Jones
Allele-Specific Oligonucleotide PCR
Elaine K. Green
Long-Range PCR,
Peter A. Davies and George Gray
Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
Anu Wartiovaara and Ann-Christine Syvänen
Cycle Sequencing of PCR Products
G. K. Surdhar
Fluorescent In Situ Hybridization
Sara A. Dyer and Elaine K. Green.
The Protein Truncation Test
Carol A. Hardy
Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch
Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli
Denaturing Gradient Gel Electrophoresis
Yvonne Wallis
Conformation-Sensitive Gel Electrophoresis
Ian J. Williams and Anne C. Goodeve
SSCP/Heteroduplex Analysis
Andrew J. Wallace
Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection
Laura Heisler and Chao-Hung Lee
Automated Genotyping Using the DNA MassArrayT Technology
Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Köster
An Introduction to Bioinformatics
Henry Brzeski
Andrea M. Guilliatt
Internal Labeling of DNA Probes
Ralph Rapley and Bimal D. M. Theophilus
End-Labeling of DNA Probes
Adrian J. Harwood
Southern Blotting of Agarose Gels by Capillary Transfer
Ralph Rapley and Ian J. Williams
Restriction Fragment Length Polymorphism
Mohammad S. Enayat
PCR: Principles, Procedures, and Parameters
Nicola Louise Jones
Allele-Specific Oligonucleotide PCR
Elaine K. Green
Long-Range PCR,
Peter A. Davies and George Gray
Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
Anu Wartiovaara and Ann-Christine Syvänen
Cycle Sequencing of PCR Products
G. K. Surdhar
Fluorescent In Situ Hybridization
Sara A. Dyer and Elaine K. Green.
The Protein Truncation Test
Carol A. Hardy
Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch
Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli
Denaturing Gradient Gel Electrophoresis
Yvonne Wallis
Conformation-Sensitive Gel Electrophoresis
Ian J. Williams and Anne C. Goodeve
SSCP/Heteroduplex Analysis
Andrew J. Wallace
Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection
Laura Heisler and Chao-Hung Lee
Automated Genotyping Using the DNA MassArrayT Technology
Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Köster
An Introduction to Bioinformatics
Henry Brzeski