Chlamydia trachomatis
Description
This detailed volume explores the state of research into the obligate intracellular bacterium, Chlamydia, in order to understand its biology and pathogenesis after the advent of genetics and new research tools. The book covers a comprehensive range of topics including infection models, imaging methods, gene expression analysis, genetic manipulation, and assays of the intracellular infection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chlamydia trachomatis: Methods and Protocols serves as a vital resource for scientists working to study this important bacterial pathogen that is a prevalent cause of human infection and disease.
Chapters 13, 22, and 23 are available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.
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Content
Monitoring Chlamydia trachomatis Development in Polarized Endocervical Epithelial Cells.- Chlamydia trachomatis Infection in a Microphysiologic Cell Culture Model.- Quantifying Chlamydia in the Mouse Gastrointestinal Tract.- Quantification of Human T Cell Responses to Chlamydia trachomatis: Intracellular Cytokine Staining (ICS) and Activation-Induced Marker (AIM) Assays.- Live Cell Imaging of Chlamydia Inclusion Membrane Proteins Using Genetically-Encoded Antibodies.- Live Imaging of Ions in Cells Infected with Chlamydia trachomatis.- Labeling and Detection of Peptidoglycan in Chlamydia and Chlamydia-Related Organisms Utilizing D-Alanine Analogs.- High-Resolution Detection of Chlamydia Proteins by Expansion Microscopy.- High-Resolution Detection of Sphingolipids during Chlamydia trachomatis Infection via Expansion Microscopy.- Genome-Wide Quantification of Protein-DNA Interactions in Chlamydia Using ChIP-Seq (Chromatin Immunoprecipitation/Deep Sequencing).- Analysis of Spatial Gene Expression in Chlamydia Using Fluorescent In Situ Hybridization.- Analysis of Chlamydia trachomatis Gene Function via Controlled Up- or Down-Regulation.- Calcium Chloride-Mediated Transformation of Chlamydia trachomatis and Other Chlamydia spp.- Generation of Chromosomal Deletions in Chlamydia trachomatis.- Chromosome Editing Using the Lambda Red Recombinase System in Chlamydia trachomatis and Chlamydia muridarum.- Construction of Inducible Transposon Mutant Libraries in Chlamydia trachomatis.- Inducible Protein Knockdown via Engineered Small RNAs in Chlamydia.- Conditional dCas12-Mediated Gene Knockdown and Complementation in Chlamydia.- A Conditional Knockout Mutant Approach to Study Essential Genes in Chlamydia in Cell Culture and In Vivo.- Disruption of Chlamydia trachomatis Essential Genes Using DOPE (Dependence on Plasmid-Mediated Expression).- Methods for Identifying Type III Secretion System Effectors in Chlamydia trachomatis.- Split-GFP Reporter-Based Detection of Chlamydia trachomatis Inclusion Damage.- Experimental Evolution of Drug Resistance for Antibiotic Target Discovery in Chlamydia trachomatis.