Essential Developmental Biology
Published on 1. August 1993
Book
Hardback
361 pages
978-0-19-963423-1 (ISBN)
Description
In recent years, developmental biology has undergone a revolution. The introduction of new techniques of molecular and cell biology, couples with those designed by classical embryologists, has provided a new approach to the study of mechanisms that control early animal development. As new techniques continue to be developed and refined, there is now an immediate need for a book that brings together approaches from many different disciplines as they apply to embryos. This volume brings together easy-to-follow protocols and practical instructions for all of the main techniques in classical embryo manipulation, from traditional embryology to cellular and molecular methods. It includes reprints of all the stage tables in common use for the main laboratory species. It continues with practical instruction on techniques for ebryological manipulations such as fate mapping, cell lineage analysis, ablation and transplantation, together with molecular methods for constructing cDNA libraries, cloning genes, analyzing their expression and disrupting their action during development. Each chapter is written by authorities in the field.
An addition to the book are reprints of the most commonly-used staging tables for zebrafish, amphibian and chick embryos. This book should provide a guide for those who wish to broaden their approach to cover different animal species, or to move across the boundaries between molecular, cellular and embryological strategies.
An addition to the book are reprints of the most commonly-used staging tables for zebrafish, amphibian and chick embryos. This book should provide a guide for those who wish to broaden their approach to cover different animal species, or to move across the boundaries between molecular, cellular and embryological strategies.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
halftones, line drawings, tables, bibliography
ISBN-13
978-0-19-963423-1 (9780199634231)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Part 1 Basic techniques - obtaining and handling embryos: drosophila embryos, Zandy Forbes and Philip Ingham; nematode embryos, Lois G. Edgar and William B. Wood; embryos and larvae of invertebrate deuterostomes, Nicholas D. Holland and Linda Z. Holland; zebrafish embryos, Rachel M. Warga; Xenopus, Ariel Ruis I. Altaba; avian embryos, Claudio D. Stern; post-implantation of mammalian embryos, Gillian M. Morriss-Kay; simple tips for photomicrography of embryos, Claudio D. Stern. Part 2 Experimental embryology: fate maps and cell lineage analysis, Ariel Ruiz I. Altaba et al; micro-injection into drosophila embryos, Philip Ingham and Zandy Forbes; cell transplantation in xenopus, Ariel Ruiz I. Altaba; transplantation in avian embryos, Claudio D. Stern; chick limb buds, Cheryll tickle; nuclear transplantation, John B. Gordon; laser ablation of cells, Judith Eisen et al. Part 3 Cellular techniques: studying cell movements in vivo by time-lapse video microscopy, Peter V. Thorogood and Dee Amanze; induction assays in Xenopus, Ariel Ruiz I. Altaba; culture of avian neural crest cells, Elisabeth Dupin and Nicole M. Le Douarin; embryonic stem cells and gene targeting, Elizabeth J. Robertson and Gail R. Martin; introduction of genes using retroviral vectors, Jack Price. Part 4 Molecular techniques: immonocytochemistry of embryonic material, Claudio D. Stern; RNAase protection assays, Ariel Ruiz I. Altaba; cDNA library construction, Akira Kakizuka et al; analysis of gene expression by reverse transcription polymerase chain reaction, Peter Koopman; cloning genes using the polymerase chain reaction, Peter W.H. Holland; in situ hybridization, David G. Wilkinson. Appendices: stage tables; suppliers of specialist items.