Genomics Protocols
Published on 10. June 2001
Book
Hardback
XIV, 538 pages
978-0-89603-774-8 (ISBN)
Description
We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of "bottom-up" and "top-down" approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27-30).
Reviews / Votes
The two editors of this volume have invited contributors from a variety of authors to write a laboratory manual dealing exclusively with the application of genomic methods in eukaryotes. Each chapter is presented in a standardized fashion, beginning with an introduction followed by a list of all the materials needed (including the machines and software) a detailed experimental protocol, notes (troubleshooting), and a list of references. it will be of value for those requiring step-by-step cookbook procedures for some of the methodologies described, and this definitive and authorative volume belongs in the library of every quality research institution where genomics is performed. - CHEMBIOCHEMMore details
Series
Language
English
Place of publication
NJ
United States
Target group
College/higher education
Professional and scholarly
Illustrations
113
5 farbige Abbildungen, 113 s/w Abbildungen
Dimensions
Height: 22.9 cm
Width: 15.2 cm
Weight
2 gr
ISBN-13
978-0-89603-774-8 (9780896037748)
DOI
10.1385/159259235X
Schweitzer Classification
Other editions
New editions
Mike Starkey | Ramnath Elaswarapu
Genomics Protocols
Book
02/2008
2nd Edition
Humana Press Inc.
€106.99
Shipment within 15-20 days
Additional editions
Michael P. Starkey | Ramnath Elaswarapu
Genomics Protocols
Book
06/2001
Humana Press Inc.
€128.39
Article exhausted; check different version
Content
Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse
Paul A. Lyons
Genetic Analysis of Complex Traits
Stephen P. Bryant and Mathias N. Chiano
Sequence-Based Detection of Single Nucleotide Polymorphisms
Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, and Mark J. Rieder
Genomic Mismatch Scanning for the Mapping of Genetic Traits
Farideh Mirzayans and Michael A. Walter
Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization
Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, and Jan P. A. Baak
Construction of a Bacterial Artificial Chromosome Library
Sangdun Choi and Ung-Jin Kim
Contiguation of Bacterial Clones
Sean J. Humphray, Susan J. Knaggs, and Ioannis Ragoussis
Mapping of Genomic Clones by Fluorescence In Situ Hybridization
Margaret A. Leversha
Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map
Panagiotis Deloukas
Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method
Yutaka Suzuki and Sumio Sugano
Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map
Panagiotis Deloukas
Preparation and Screening of High-Density cDNA Arrays with Genomic Clones
Günther Zehetner, Maria Pack, and Katja Schäfer
Direct Selection of cDNAs by Genomic Clones
Daniela Toniolo
Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System
Martin C. Wapenaar and Johan T. Den Dunnen
Sequencing Bacterial Artificial Chromosomes
David E. Harris and Lee Murphy
Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics
Yvonne J. K. Edwards and Simon M. Brocklehurst
Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning
GregElgar
Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization
Oliver Dorian von Stein
Isolation of Differentially Expressed Genes by Representational Difference Analysis
Christine Wallrapp and Thomas M. Gress
Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display
Michael P. Starkey
Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos
Nicolas Pollet and Christof Niehrs
Expression Monitoring Using cDNA Microarrays: A General Protocol
Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn, and Ronald W. Davis
Prediction of Protein Structure and Function by Using Bioinformatics
Yvonne J. K. Edwards and Amanda Cottage
Identification of Novel Genes by Gene Trap Mutagenesis
Anne K. Voss and Tim Thomas
Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice
Ahmed Mansouri
Genomic Analysis Utilizing the Yeast Two-Hybrid System
Ilya G. Serebriiskii, Garabet G. Toby, Russell L. FInley, Jr., and Erica A. Golemis
Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle
Terry J. Amiss and Richard Jude Samulski
Retroviral-Mediated Gene Transduction
Donald S. Anson
Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods
Ruth Gjerset, Ali Haghighi, Svetlana Lebedeva, and Dan Mercola
Ribozyme Gene Therapy
Leonidas A. Phylactou
Paul A. Lyons
Genetic Analysis of Complex Traits
Stephen P. Bryant and Mathias N. Chiano
Sequence-Based Detection of Single Nucleotide Polymorphisms
Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, and Mark J. Rieder
Genomic Mismatch Scanning for the Mapping of Genetic Traits
Farideh Mirzayans and Michael A. Walter
Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization
Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, and Jan P. A. Baak
Construction of a Bacterial Artificial Chromosome Library
Sangdun Choi and Ung-Jin Kim
Contiguation of Bacterial Clones
Sean J. Humphray, Susan J. Knaggs, and Ioannis Ragoussis
Mapping of Genomic Clones by Fluorescence In Situ Hybridization
Margaret A. Leversha
Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map
Panagiotis Deloukas
Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method
Yutaka Suzuki and Sumio Sugano
Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map
Panagiotis Deloukas
Preparation and Screening of High-Density cDNA Arrays with Genomic Clones
Günther Zehetner, Maria Pack, and Katja Schäfer
Direct Selection of cDNAs by Genomic Clones
Daniela Toniolo
Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System
Martin C. Wapenaar and Johan T. Den Dunnen
Sequencing Bacterial Artificial Chromosomes
David E. Harris and Lee Murphy
Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics
Yvonne J. K. Edwards and Simon M. Brocklehurst
Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning
GregElgar
Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization
Oliver Dorian von Stein
Isolation of Differentially Expressed Genes by Representational Difference Analysis
Christine Wallrapp and Thomas M. Gress
Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display
Michael P. Starkey
Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos
Nicolas Pollet and Christof Niehrs
Expression Monitoring Using cDNA Microarrays: A General Protocol
Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn, and Ronald W. Davis
Prediction of Protein Structure and Function by Using Bioinformatics
Yvonne J. K. Edwards and Amanda Cottage
Identification of Novel Genes by Gene Trap Mutagenesis
Anne K. Voss and Tim Thomas
Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice
Ahmed Mansouri
Genomic Analysis Utilizing the Yeast Two-Hybrid System
Ilya G. Serebriiskii, Garabet G. Toby, Russell L. FInley, Jr., and Erica A. Golemis
Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle
Terry J. Amiss and Richard Jude Samulski
Retroviral-Mediated Gene Transduction
Donald S. Anson
Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods
Ruth Gjerset, Ali Haghighi, Svetlana Lebedeva, and Dan Mercola
Ribozyme Gene Therapy
Leonidas A. Phylactou