Practical HPLC Method Development
2nd Edition
Published on 27. March 1997
Book
Hardback
800 pages
978-0-471-00703-6 (ISBN)
Description
This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.
More details
Edition
2. Auflage
Language
English
Place of publication
United States
Publishing group
John Wiley & Sons Inc
Target group
Professional and scholarly
Edition type
New edition
Product notice
sewn/stitched
Cloth over boards
Dimensions
Height: 240 mm
Width: 161 mm
Thickness: 47 mm
Weight
1343 gr
ISBN-13
978-0-471-00703-6 (9780471007036)
Schweitzer Classification
Other editions
Additional editions
Lloyd R. Snyder | Joseph J. Kirkland | Joseph L. Glajch
Practical HPLC Method Development
E-Book
12/2012
2nd Edition
Wiley
€194.99
Available for download
Previous edition
Lloyd R. Snyder | J.L. Glajch | J.J. Kirkland
Practical HPLC Method Development
Book
11/1988
Wiley
€87.84
Article exhausted; check for reprint
Persons
Lloyd R. Snyder, PHD, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, and the bestselling Practical HPLC Method Development, Second Edition, all published by Wiley.
Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley.
Joseph L. Glajch is the author of Practical HPLC Method Development, Second Edition, published by Wiley.
Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley.
Joseph L. Glajch is the author of Practical HPLC Method Development, Second Edition, published by Wiley.
Content
Getting Started.
Basics of Separation.
Detection Sensitivity and Selectivity.
Sample Preparation.
The Column.
Non-Ionic Samples: Reversed- and Normal-Phase HPLC.
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.
Gradient Elution.
Systematic Approach to the Reversed-Phase Separation of Regular Samples.
Computer-Assisted Method Development.
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
Chiral Separations.
Preparative HPLC Separation.
Quantitation (Including Trace Analysis).
Completing the Method: Validation and Transfer.
Appendices.
Index.