RNA-Protein Interactions
Published on 1. September 1998
Book
Hardback
320 pages
978-0-19-963651-8 (ISBN)
Description
RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly-catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. This text provides a guide to the experimental procedures used in studying RNA-protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in-depth analysis of the structural basis of the interaction.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
20 halftones, 41 line figures
ISBN-13
978-0-19-963651-8 (9780199636518)
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Schweitzer Classification
Content
Applications of chemically-synthesized RNA; preparation of RNA-protein complexes for x-ray crystallography and NMR; uses of site-specifically modified RNAs constructed by RNA ligation; the electrophoretic mobility shift assay for RNA binding proteins; affinity methods for isolating RNA-binding proteins; synthetic lethal/enhancer screening to identify snRNA-protein and protein-protein interactions in yeast pre-mRNA splicing; detecting RNA-protein interactions and isolating cDNA clones by northwestern screening; a three-hybrid system to detect and analyze RNA-protein interactions in vivo; in-vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay; foot-printing and modification-interference analysis of binding sites on RNA; analysis of RNA-protein and RNA-RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques; in-vitro selection of nucleic acid ligands.