Ancient DNA
Methods and Protocols
2nd Edition
Published on 15. March 2019
Book
Hardback
X, 216 pages
978-1-4939-9175-4 (ISBN)
Description
This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab.
Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab.
More details
Series
Edition
Second Edition 2019
Language
English
Place of publication
New York
United States
Target group
Professional and scholarly
Illustrations
10 s/w Abbildungen, 5 farbige Abbildungen
X, 216 p. 15 illus., 5 illus. in color.
Dimensions
Height: 260 mm
Width: 183 mm
Thickness: 18 mm
Weight
634 gr
ISBN-13
978-1-4939-9175-4 (9781493991754)
DOI
10.1007/978-1-4939-9176-1
Schweitzer Classification
Other editions
Additional editions
E-Book
03/2019
2nd Edition
Humana
€160.49
Available for download
Content
Setting Up an Ancient DNA Laboratory.- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate.- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step.- Extraction of Highly Degraded DNA from Ancient Bones and Teeth.- Sampling and Extraction of Ancient DNA from Sediments.- Extraction of Ancient DNA from Plant Remains.- DNA Extraction from Keratin and Chitin.- Double-Stranded Library Preparation for Ancient and Other Degraded Samples.- A Method for Single-Stranded Ancient DNA Library Preparation.- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms.- Whole-Genome Capture of Ancient DNA Using Homemade Baits.- Generating RNA Baits for Capture-Based Enrichment.- Hybridization Capture of Ancient DNA Using RNA Baits.- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA.- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis.- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA.- Authentication and Assessment of Contamination in Ancient DNA.- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence.