
Protein Purification
Principles and Practice
Robert K. Scopes(Author)
Springer (Publisher)
3rd Edition
Published on 19. November 1993
Book
Hardback
XX, 380 pages
978-0-387-94072-4 (ISBN)
Description
New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
Reviews / Votes
From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)
More details
Series
Edition
Third Edition 1994
Language
English
Place of publication
New York
United States
Target group
Primary & secondary/elementary & high school
Graduate
Edition type
New edition
Illustrations
XX, 380 p.
Dimensions
Height: 23.4 cm
Width: 15.6 cm
Weight
1640 gr
ISBN-13
978-0-387-94072-4 (9780387940724)
DOI
10.1007/978-1-4757-2333-5
Schweitzer Classification
Other editions
Additional editions

E-Book
06/2013
3rd Edition
Springer
€96.29
Available for download

Book
12/2010
3rd Edition
Springer
€96.29
Shipment within 15-20 days
Content
1 The Protein Purification Laboratory.- 2 Making an Extract.- 3 Analysis-Measurement of Protein and Enzyme Activity.- 4 Separation by Precipitation.- 5 Separation by Adsorption I: General Principles.- 6 Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents.- 7 Separation by Adsorption-Affinity Techniques.- 8 Separation in Solution.- 9 Purification of Special Types of Proteins.- 10 Small-Scale and Large-Scale Procedures.- 11 Analysis for Purity.- 12 Optimization of Procedures; Final Steps.- Appendix A.- Precipitation Tables.- Appendix B.- Solutions for Measuring Protein Concentration.- Appendix C.- Buffers for Use in Protein Chemistry.- Appendix D.- Chromatographic Materials.- References.