
Laboratory Manual of Microbiology
New India Publishing Agency
Published on 15. January 2010
Book
Paperback/Softback
210 pages
978-81-19215-77-5 (ISBN)
Description
The book in question comprises 15 chapters, each focusing on a specific aspect of microbiology. These chapters include techniques for isolating microbes from various sources, such as soil, water, and seeds, as well as methods for assessing microbial growth and sensitivity to antibiotics. Additionally, the book covers topics such as bacterial transformation, fermentation, and microbial analysis of air, soil, and water. The authors have designed the book to provide laboratory techniques for undergraduate and postgraduate students to complete assignments in their course syllabi. To aid in understanding, many of the methods are illustrated with flowcharts and diagrams. The authors are confident that the book will be a valuable resource for those seeking to learn and perform microbiological experiments in the laboratory.
More details
Series
Language
English
Place of publication
New Delhi
India
Target group
Professional and scholarly
Weight
410 gr
ISBN-13
978-81-19215-77-5 (9788119215775)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
A.K. Roy: Professor and Head, Course Coordinator, P.G. Department of Biotechnology, T.M. Bhagalpur, University, Bhagalpur, Bihar,India.
M.M. Prasad: Professor, University Department of Botany, T.M. Bhagalpur, University, Bhagalpur, Bihar,India
M.M. Prasad: Professor, University Department of Botany, T.M. Bhagalpur, University, Bhagalpur, Bihar,India
Content
1. Organization of microbiology laboratory.
2. Preparation of media-liquid and solid for growth of microorganisms.
3. Isolation techniques and maintenance of microorganisms.
4. Isolation of pure cultures from soil, air, water and seed surface.
5. Staining and microscopic examination of microbes.
6. Micrometry and measurement of spores.
7. Microbial growth.
8. Study of mutations by Ames test.
9. Assay of antibiotics, antibiotics sensitivity of microbes and demonstration of antibiotic resistance.
10. Bacterial transformation.
11. Biochemical characterization of enteric bacteria (IMVIC test).
12. Isolation and one step growth curve of coliphage.
13. Testing of water quality (bacteriological examination of water).
14. Culture from body fluids (urine, stool, blood).
15. Mixed acid and alcoholic fermentation.
2. Preparation of media-liquid and solid for growth of microorganisms.
3. Isolation techniques and maintenance of microorganisms.
4. Isolation of pure cultures from soil, air, water and seed surface.
5. Staining and microscopic examination of microbes.
6. Micrometry and measurement of spores.
7. Microbial growth.
8. Study of mutations by Ames test.
9. Assay of antibiotics, antibiotics sensitivity of microbes and demonstration of antibiotic resistance.
10. Bacterial transformation.
11. Biochemical characterization of enteric bacteria (IMVIC test).
12. Isolation and one step growth curve of coliphage.
13. Testing of water quality (bacteriological examination of water).
14. Culture from body fluids (urine, stool, blood).
15. Mixed acid and alcoholic fermentation.