
Lymphocytes
A Practical Approach
Oxford University Press
2nd Edition
Published on 16. December 1999
Book
Hardback
376 pages
978-0-19-963817-8 (ISBN)
Article exhausted; check different version
Description
Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the
generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification
assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification
assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
Reviews / Votes
"... the book contains extremly useful practical advice for studying lymphocytes in the laboratory..." Parasitology TodayMore details
Series
Edition
2nd Revised edition
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Edition type
Revised edition
Illustrations
numerous illustrations (some colour)
Dimensions
Height: 241 mm
Width: 161 mm
Thickness: 24 mm
Weight
765 gr
ISBN-13
978-0-19-963817-8 (9780199638178)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions

Book
12/1999
2nd Edition
Oxford University Press
€69.33
Shipment within 15-20 days
Previous edition
Book
11/1987
Oxford University Press
€57.14
Article exhausted; check for reprint
Persons
Editor
Institute of Molecular Medicine, Oxford
Institute of Molecular Medicine, Oxford
Content
Preface ; Preparation of lymphocytes and idenfication of lymphocyte subpopulations ; Immunohistochemistry of lymphoid organs ; T and B-cell hybridomas ; Murine T-cell culture ; Human CD4+ T-cell culture ; Human Cytotoxic T-cell culture ; Limiting dilution analysis for quantification of antigen-specific T-cells ; HLA -peptide tetrameric complexes ; Expansion of human T-cells for immunotheraphy ; HLA typing ; Characterisation of lymphocyte surface markers ; Apoptosis assays for lymphocytes ; Thymic organ culture ; Index