High Performance Liquid Chromatography in Enzymatic Analysis

The primary focus of this book is on the use of high pressure liquid chromatography (hplc) as a technique for the determination of enzyme activitities. Basic concepts and principles are treated early in the volume followed by a consideration of the strategies to be employed for the determination of activities for a wide variety of enzymes from diverse tissues, body fluids and individual cells. Of particular value are the large number (over 170) of illustrations reproduced from the original research literature. The final chapter treats the assay of multienzyme systems, an area of emerging importance in the study of the mechanisms of enzyme action.