Methods in Cell Biology: Flow Cytometry v.42
Academic Press
2nd Edition
Published on 29. October 1997
Book
Hardback
627 pages
978-0-12-564143-2 (ISBN)
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Description
This is the second of a two volume set that provides a laboratory guide and reference on methods in flow cytometry sample preparation and analysis. The theoretical foundations, critical aspects and possible pitfalls of each procedure is outlined, and protocols are supplemented with explanatory sections and trouble-shooting tips. Various cell systems are described and ten methods cover assessment of apoptosis and other modes of cell death. The methods detailed should be accessible to researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells.
More details
Series
Edition
2nd Revised edition
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
College/higher education
Professional and scholarly
Edition type
Revised edition
Illustrations
index
Dimensions
Height: 235 mm
Width: 199 mm
Weight
1497 gr
ISBN-13
978-0-12-564143-2 (9780125641432)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
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Persons
Editor
Purdue University, West Lafayette, Indiana,USA
Biomedical Sciences Division, Los Alamos National Laboratories, New Mexico,USA
Volume editor
Content
A flow cytometric technique for detection of DNA repair in mammalian cells, J.R. Selden and F. Dolbeare; rapid determination of cellular resistance related drug efflux in tumour cells, A. Krishan; glutathione and cellular resistance to anti-cancer drugs, D. Hedley and S. Chow; assay of cell resistance to Ara-C, F. Lacombe; detection of mRNA species by flow cytometry, F. Belloc; PRimed IN Situ labelling (PRINS) and Fluorescent In Situ Hybridization (FISH), H. Fischer et al; molecular phenotyping by flow cytometry, B.D. Li et al; isolation and analysis of somatic cell mutants with defects in endocytic traffic, S.A. Brockman and R.F. Murphy; measurement of micronuclei by flow cytometry, M. Nusse et al; sperm chromatin structure assay (DNA denaturability), D. Evenson and L. Jost; fine-needle cytopuncture and flow cytometry in the characterization of human tumours, F. Spyratos and M. Briffod; functional NK assay using flow cytometry, S. Papa and M. Valentini; antibodies to intermediate filament proteins as probes for multi-parameter flow cytometry of human cancers, T.V. Shankey; interactive data analysis for evaluation of B-cell neoplasia by flow cytometry, R.E. Duque; analysis and sorting of hematopoietic stem cells from mouse bone marrow, J.M.W. Visser and P. de Vries; reticulocyte analysis and reticulocyte maturity index, B.H. Davis and N.C. Bigelow; analysis of platelets by flow cytometry, K.A. Ault and J. Mitchell; flow cytometry in malaria detection, C.J. Janse and P.H. Van Vianen; large-scale chromosomes sorting, J.J. Fawcett et al; strategies for rare cell detection and isolation, J.F Leary; cell sorting of biohazardous specimens for assay of immune function, J.V. Giorgi; high-speed photodamage cell sorting - an evaluation of the ZAPPER prototype, J.F. Keij et al; high-gradient magnetic cell sorting, A. Radbruch et al; contributions of flow cytometry to studies with multicell spheroids, R.E. Durand; functional measurements using HL-60 cells, J.P. Robinson et al; HIV infection - diagnosis and disease progression evaluation, J.V. Giorgi and A. Landay; cell cycle analysis of "saccharomyces cerevisiae", B.S. Dien et al; staining and measurement of DNA in bacteria, H.B. Steen et al; detection of specific microorganisms, G. Vesey et al; strategies for flow cytometric analysis of marine microalgae and sponge cells, C.M. Yentsch and S.A. Pomponi; flow cytometry and sorting of plant protoplasts and cells, D.W. Galbraith; use of fluorescence-activated cell sorting for rapid isolation of insect cells harbouring recombinant baculovirus, M.A. Sommerfelt and E.J. Sorscher; flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes, T.M. McHugh; calibration of flow cytometer detector systems, R.E. Durand. (Part contents).