Neoplastic Transformation in Human Cell Culture

I. Preneoplastic Events.- Comparison of human versus rodent cell transformation: importance of cell aging.- Deficient DNA repair, an early step in neoplastic transformation of human cells in culture.- Use of immortalized human keratinocytes for the study of squamous differentiation and mutagenesis.- Studies of mutagen-activated genes which confer anchorage-independence: the c-sis gene as a model.- Cytoskeletal changes in human transformed cells: Studies on HOS cells.- Polyamine metabolism in human epidermal keratinocytes transformed with AD12-SV40, HPV16-DNA, and K-ras oncogene.- II. Radiation Transformation and Oncogenes.- Transformation of human diploid fibroblasts by radiation and oncogenes.- Ionizing radiation-mediated protein kinase C activation and gene expression.- Detection of transforming genes from radiation transformed human epidermal keratinocytes by a tumorigenicity assay.- Neoplastic transformation of human epithelial cells by ionizing radiation.- Effects of ionizing radiation on human papillomavirus immortalized human bronchial epithelial cells.- Biochemical purification of a CSF-1 like molecule released during malignant transformation of IL-3 dependent hematopoietic progenitor cell lines cocultivated with gamma irradiated clonal marrow stromal cell lines.- An inherited p53 point mutation in a cancer prone family with Li-Fraumeni syndrome.- p53, a direct target of mutational activation by chemical carcinogens?.- III. Viral Transformation and Oncogenes.- The HIV tat gene induces epidermal hyperplasia in vivo and transforms keratinocytes in vitro.- Immortalization and tumorigenic transformation of normal human cervical epithelial cells transfected with human papillomavirus DNAs.- Using the papillomavirus E6/E7 genes to generate well-differentiated epithelial cell lines.- Tumor progression in breast cancer.- Growth regulation of HPV-positive keratinocytes by TGF-01.- Cell growth transformation by Epstein Barr virus.- Isolation and characterization of a transformation-associated gene from human nasopharyngeal carcinoma cells.- Molecular pathogenesis of lung cancer: Mutations in dominant and recessive oncogenes and the expression of opioid and nicotine receptors in the pathogenesis of lung cancer.- Molecular control of expression of plasticity of tumorigenic/ metastatic phenotypes.- IV. Multistep Models.- Altered regulation of growth and differentiation at different stages of transformation of human skin keratinocytes.- Neoplastic transformation and suppression of transformation of human bronchial epithelial cells in vitro.- An in vitro human mammary epithelial model system for studies of differentiation and carcinogenesis.- Transformation in vitro of human uroepithelial cells.- Multiple steps in the in vitro immortalization and neoplastic conversion of human colonic epithelial cells.- A human renal epithelial multistep model of in vitro carcino-genesis.- Immortalization and oncogene transformation of human esopha-geal epithelial cells.- A nontumorigenic human liver epithelial cell culture model for chemical and biological carcinogenesis investigations.- Establishment and characterization of SV40 T-antigen immortal-ized human liver cells.- Transformation of human tracheal gland epithelial cells in vitro.- Stable expression of SV40 large T-antigen gene in primary human Schwann cells.- Malignant transformation of human fibroblasts in vitro.- Mitogen-independence and autocrine growth factor secretion displayed by human mesothelioma cells and oncogene-transfected mesothelial cells.- Structure and growth regulation in normal, Transformed and malignant human endometrial cell cultures.- Construction of a unidirectional cDNA library from a radio-resistant laryngeal squamous cell carcinoma cell line in an Epstein Barr virus shuttle vector.- Contributors.- Registrants.