Bioanalytics for Beginners
Published on 31. March 2012
Book
Hardback
428 pages
978-1-4614-0922-9 (ISBN)
Description
Modeled in its aims and graphic presentation on the supremely successful manual Biotechnology for Beginners, this book is angled at students and higher-education instructors of medicine, biotechnology, biochemistry, chemistry and the ecological sciences; medical technologists; lab technicians; biology and chemistry teachers; and readers of Scientific American looking for a readily understandable introduction to biological analysis methods and their applications. Largely speaking, it deals with bioanalytic facts, examining the way they interconnect and the background knowledge required to understand them, yet it also discusses the economic and ethical pros and cons involved. The text is easy to read and strongly geared to teaching the subject. Further, the wide range of pictorial features makes it especially interesting and entertaining. The layout is based on that of Biotechnology for Beginners. This manual is an excellent preparation for the Spektrum book on bioanalysis as well as the experimentation volumes from the same publisher.
More details
Edition
2012
Language
English
Place of publication
New York, NY
United States
Target group
College/higher education
Upper undergraduate
Illustrations
414 farbige Abbildungen, 27 s/w Abbildungen
27 black & white illustrations, 414 colour illustrations
Dimensions
Height: 260 mm
Width: 155 mm
ISBN-13
978-1-4614-0922-9 (9781461409229)
DOI
10.1007/978-1-4614-0923-6
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Bild-DVD, Bioanalytik für Einsteiger
Alle Grafiken des Buches
Software
12/2008
1st Edition
Spektrum Akademischer Verlag
€25.00
Shipment within 7-9 days
Person
Reinhard Renneberg has been a Professor of Analytic Biotechnology at the Hong Kong University of Science and Technology (www.ust.hk) since 1955. He is the author of the extremely successful and much-lauded volume Biotechnology for Beginners, of which over 5,300 copies have been sold in 18 months. He has also written three other books on biotechnology, including Katzenklon. He is a co-author of the Roempp Biotechnology Dictionary, has written four monographs and 250 publications and holds 20 patents. In addition, he is involved in two biotechnology companies in Germany and China.
Content
PREFACE CHAPTER 1 The Nanoru - The incredible story of its isolation, purification, and characterization 1.1 The Site 1.2 Rearing and pure culture 1.3 Biomass production 1.4 Activity Test 1.5 Gel and ion exchange chromatography 1.6 Affinity chromatography 1.7 Isoelectric focusing 1.8 Gel electrophoresis 1.9 Mass and sequence analysis 1.10 How the Gen was "fished" 1.11 X-ray structure and NMR 1.12 The Sensation: The Nanoru - sudden clarity 1.13 How do we continue with the Nanoru? CHAPTER 2 Biomolecules in tests - Instrumental Bioanalytics 2.1 More and more machines for ever-smaller particles 2.2 Protein separation 2.3 Gel filtration chromatography separates proteins by size 2.4 Ion exchange chromatography separates proteins after their charge 2.5 Molecular Ying and Yang: affinity chromatography 2.6 High-performance liquid chromatography (HPLC) 2.7 What occurs with purification? Electrophoresis analyses protein mixtures qualitatively 2.8 Isoelectric focusing separates proteins according to their neutral points 2.9 Capillary electrophoresis combines high selectivity with brief periods of separation 2.10 Antibody probes identify proteins 2.11 The instrumental exploration of protein structure 2.12 Edman - Sequencing deciphered the primary structure of a protein 2.13 Mass spectrometry determined exact protein and peptide primary structure 2.14 X-ray structure deciphered protein conformation 2.15 The nuclear resonance spectroscopy (NMR) studied proteins in solution CHAPTER 3 Biocatalysis - enzymes and enzyme tests 3.1 Enzymes: highly specific and efficient molecular machines 3.2 Chicken or egg? Ribozymes are also biocatalysts 3.3 How enzymes recognize substrates 3.4 How enzymes are named and classified 3.5 Key-lock or hand glove? 3.6 Coenzymes are transformed like substrates 3.7 Enzyme kinetics: how enzyme reactions are in the time progress 3.8 Unit and Katal: the measurement of enzyme activity 3.9 Getting started: optical enzyme tests 3:10 Dry chemistry: from the litmus test paper for glucose-testing 3.11 Inhibition of enzyme reactions 3.12 Bird dead or!: the exact measurement of enzyme inhibitors 3.13 Isoenzymes 3.14 Enzyme-activity tests CHAPTER 4 Bio-affinity I: antibodies and immune tests 75 4.1 Is War vaccination successful? The ring test 4.2 How antigens and antibodies react with Haptene 4.3 Blood is a very special juice: determination of blood groups 4.4 Soluble + soluble = insoluble: immune precipitation 4.5 Diffusion combined with electrophoresis: immune electrophoresis 4.6 Useful protein blurs: Western blotting 4.7 Nephelometry: to fish in the dark with success 4.8 Immuno assays: "The best [perfect] is the enemy of the good" 4.9 Thyroid tests with the Radio immuno assay 4.10 Immunology with the power of enzymes: ELISA 4.11 Indirect ELISA: detection of antibodies against HIV and the Dot test 4.12 Rapid immuno-tests: a baby -- yes or no? 4.13 The rapid detection of a death angel: HIV tests 4.14 Quick help with heart attacks 4.15 A worldwide trend: Point of care (POC) tests 4.16 Drugs and their abuse 4.17 Immunological drug tests 4.18 How to evaluate a laboratory test: sample HIV testing 4.19 How to test tests: ROC-curves CHAPTER 5 Bio-affinity II: biological receptors - nature as unsurpassed Bioanalysis lab worker 5.1 The fantastic dog nose: A million-times better than ours! 5.2 Our human meaning 5.3 Smell: olfactory detection 5.4 How does a receptor work? 5.5 Electronic nose: Combined polymers versus real receptors 5.6 Taste: gustatory detection 5.7 Visual detection 5.8 The evolution of the eye 5.9 Processes in the retina 5.10 Seeing colours 5.11 Listen: acoustic detection 5.12 Molecular mechanisms of hearing 5.13 Touch: tactile detection 5.14 Are there any other sensory systems? CHAPTER 6 DNA, RNA, and their amplification 6.1 DNA: the double helix 6.2 Rools for DNA analysis: DNA polymerase 6.3 DNA to RNA: RNA polymerase 6.4 In short: the DNA code 6.5 Structural genes, exons and introns 6.6 Plasmid as a DNA cuckoo 6.7 DNA scissors and glue: restriction endonucleases and ligases 6.8 Inverted world: RNA into DNA by reverse transcriptase 6.9 How to gain nucleic acids 6.10 Experiment! DNA isolated from zucchini in the kitchen 6.11 Determination of optic concentration of nucleic acids 6.12 DNA probes to detect DNA 6.13 How to analyze DNA: gel electrophoresis separates DNA fragments according to their size 6.14 Life and death: genetic fingerprints to detect paternity or murder 6.15 DNA markers: tandems and "snippets" 6.16 The polymerase chain reaction: DNA copier 6.17 Reverse transcriptase-PCR for the detection of RNA viruses 6.18 The real-time PCR (RTQ-PCR) quantifies PCR products 6:19 How genes are sequenced 6.20 Southern blotting 6:21 Automatic DNA sequencing 6.22 FISH: chromosomal location and number of gene copies CHAPTER 7 Biosensors 7.1 Enzyme tests for millions of diabetics 7.2 Diabetes mellitus - what to do? 7.3 Glucose biosensors: highly effective combinations of biomolecules and sensors 7.4 Glucose-test yourself! 7.5 Environmental control by microbial respirometry: the BOD5 test 7.6 Cell sensors measure the sewage pollution in five minutes 7.7 Molecular dog hunting: Piezoelectric sensors 7.8 Mirage, optical sensors, and BIAcore 7.9 Real-time measurements with SPR 7.10 How can one detect deadly substances with antibodies? 7.11 How does one generate a signal? 7.12 How do the antibodies of immunosensors remain functional? 7.13 biosensors with immobilized antibodies 7.14 Biochips: Targeted molecular fishing in the dark 7.15 How does a DNA chip work? 7.16 How to figure out morbidity dates and diagnose viruses 7.17 DNA chips for cytochrome P450 7.18 DANN chips for green genetic engineering, ecology, and forensics 7.19 Protein chips CHAPTER 8 Pharmaceuticals on the bench 8.1 Bioanalysis conquers drug labs 8.2 From green to red-gold vein 8.3 High-throughput drug screening for active drug candidates 8.4 New HPLC screening techniques 8.5 RP-HPLC coupled with nuclear resonance spectroscopy (NMR) 8.6 Matrix-based Laser Desorption Ionisation Mass Spectrometry (MALDI-MS) 8.7 Electrospray Ionisation Mass Spectrometry (ESI-MS) 8.8 New in vitro and in vivo tests by genetically modified organisms (GMOs): finally, the answers--or more questions? 8.9 Consideration of recombinant pharmaceuticals INSERT: BIOANALYSIS - an independent science: a timetable on the first and last book pages GLOSSARY CREDITS INDEX