Protein Engineering
Published on 1. December 1992
Book
Hardback
422 pages
978-0-19-963139-1 (ISBN)
Description
Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specifities, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. It is a practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation and biochemistry.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
halftones, numerous line drawings, tables, bibliography
ISBN-13
978-0-19-963139-1 (9780199631391)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
E.Y. Jones & D.I. Stuart: Methods of structural analysis of proteins - part 1 - protein crystallography; G.M. Clore & A.M. Gronenborn: Methods of structural analysis of proteins - part 2 - nuclear magnetic resonance; M.D.S. Boguski, J. Ostell, & D.J. States: Molecular sequence databases and their uses; C. Sander, M. Scharf, R. Schneider: Design of protein structures; P.A. Bates & M.J.E. Sternberg: From protein sequence to structure; V. Daggett & P.A. Kollman: The use of theoretical methods in protein engineering; B.E. Finn, X. Chen, P.A. Jennings, S.M. Saalau-Bethell & C.R. Matthews: Principles of protein stability - part 1 - reversible unfolding of proteins: kinetic and thermodynamic analysis; R. Wetzel: Principles of protein stability - part 2 - enhanced folding and stabilization of proteins by suppression of aggregation in vitro and in vivo; S. Jentsch & A. Bachmair: Principles of protein turnover: possible manipulations; R.E. Offord: Chemical approaches to expression of proteins; K.L. Hilyard, D. Staunton, A.E. Jones, & A.R. Rees: Protein engineering of antibody combining sites; R.H. Jackson, J. McCafferty, K.S. Johnson, A.R. Pope, A.J. Roberts, D.J. Chiswell, T.P. Clackson, A.D. Griffiths, H.R. Hoogenboom, & G. Winter: Selection of variants of antibodies and other protein molecules using display on the surface of bacteriophage; G.T. Yarranton & A. Mountain: Expression of proteins in prokaryotic systems - principles and case studies; M. Kikuchi & M. Ikehara: Expression of proteins in yeast; M. Kriegler: Genetic engineering of the processing and secretory pathway of an inflammatory cytokine in mammalian cells: a case study; R. Bischoff, M. Courtney, & D. Speck: Industrial applications of protein engineering and scale-up of protein production: l-antitrypsin, a case study.