Immunoassays
Description
This textbook provides an accessible and comprehensive introduction to the basic principles of immunological methods. It offers a fundamental insight into the performance of different immunoassays and their areas of application.
Each chapter in Immunoassays begins with a basic introduction to the topic before going on to cover important details for everyday laboratory work and professional practice. Readers will gain confidence in using the methods as potential problems are discussed in detail and troubleshooting sections help to solve them. The book covers all the classic and essential techniques, such as enzyme-linked immunosorbent assay (ELISA), dot blot, and western blot, as well as the latest advances in polymerase chain reaction (PCR), flow cytometry, fluorescence microscopy, and 3D cell and organoid culture. Importantly, the book also covers regulatory requirements and provides information on quality systems with various mandatory guidelines and regulations, such as in-vitro diagnostics, medical devices, the pharmaceutical industry, and food analysis.
Packed with many practical tips, this textbook is an invaluable resource for beginners and experienced practitioners alike. It is aimed at researchers and students in the life sciences and biomedicine, as well as laboratory assistants.
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Dr. Arnold Maria Raem works at the Centre for Nanotechnology at University Münster, Germany, in the field of molecular biomedicine/oncology.
Dr. Peter Rauch is Managing Director of Candor Bioscience GmbH in Germany, a biotechnology company involved in the research and development of products for immunoassays.
Content
Forewords.- Introduction to Immunoassays.- Antibodies.- ELISA/EIA/FIA.- Sample Preparation for Immunoassays.- Lateral Flow Immunoassays.- Immuno-PCR: Application-oriented highly sensitive protein analytics with antibody-DNA conjugates.- Evolution of PCR - from classical PCR to digital PCR.- Detection of Proteins on Membranes: Western Blot and Dot Blot Methods.- Acceleration of Western Blotting.- Lab-on-a-Chip - Miniaturization of Assays for On-Site Diagnostic Applications Using Microfluidics.- Reliable protein quantification by surface plasmon resonance spectroscopy.- Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs.- Detection Methods for Protein Interaction.- Immunohistochemistry for Research and Diagnostic Pathology.- Fluorescence Microscopy in Immunoassay Application - An Introduction.- In Vivo Immunostaining and Intravital Two-Photon Microscopy.- Quantitative digital holographic phase contrast microscopy - biophysical characterization of immunological processes, cells and tissues.- Circulating Tumor Cells - Isolation and Molecular Characterization.- Automated isolation of circulating tumor cells for liquid biopsy.- 3D Cell and Organoid Culture.- Tissue Engineering and Regenerative Medicine.- The CRISPR/Cas Technology: Classic and Future Applications in Molecular Biology and Medicine.- Fluorescent Dyes.- Stabilization of Assay Components.- Interference Effects in Immunoassays.- Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs.- Evaluation and Validation.- Food and Feed Analysis.- Automation of Immunoassays.- Challenges for a Next Generation Biobanking - Cryopreservation for the advancement of personalized medicine.- The new EU Regulation 2017/746 on in vitro diagnostic medical devices.- ISO 13485 - the mandatory quality system for IVD manufacturers.- Clinical Trials for In-vitro Diagnostics.- Protection for Diagnostic Methods and its Limits under the European Patent Convention and the German Patent Law.- Learning Laboratory Systems: How can artificial intelligence support laboratory work?.- Impedance-based Analysis of Functional Immunoassays using Cultured Cells.- Measurement of Single Molecules and Cells with Atomic Force Microscopy.- Immunoassays for Extracellular Vesicles-based Characterization.- Immunoassay Fabrication and Device Functionalization by Scanning Probe Lithography.- The digital lab of the future, laboratory information systems, analytics and AI in LifeScience R&D.- Regulation of Medical Devices in the USA with a Focus on IVDs.- Optical Coherence Tomography for Non-Invasive Imaging of Tissue and Organoids.- sFIDA: Quantitative Single Particle Analysis.- The Blood-Brain Barrier.- The Biomedical Lab, Ethics, and Obtaining Ethics Approval for Research.- A label-free future: digid´s BioMEMS platform enables diagnostics beyond the laboratory.- Ethical Boundaries of Research Freedom.- New Approaches in Personalized Tumor Therapy Research: Organoids, Organoid-on-Chip, and Tumor Explants as Models.