Free Radicals
Neville A. Punchard(Author)
Oxford University Press
Published on 1. September 1996
Book
Hardback
334 pages
978-0-19-963560-3 (ISBN)
Description
This volume brings together practical techniques for detecting and measuring free radicals - the reactive, short-lived molecules now known to play an important role in a large number of human diseases, including heart disease and cancer - and for studying their effects. Consequently, their study in biological systems has increased enormously in recent years, and this book may be of interest to a wide cross-section of biochemists and clinicians.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
halftone and line figures, tables, bibliography
ISBN-13
978-0-19-963560-3 (9780199635603)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Introduction. 1: Direct and indirect measurements of free radicals by physico-chemical methods. 1.1: In vitro and in vivo detection of free radicals metabolites with electron spin resonance. 1.2: The application of high-field nuclear magnetic resonance spectroscopy to the detection of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs. 2: Direct and indirect measurement of free radicals by other methods. 2.1: Intrinsic (low-level) chemiluminescence. 2.2: Visual assessment of oxidative stress by multifunctional digital microfluorography. 2.3: Salicylic acid and phenylalanine as probes to detect hydroxyl radicals. 3: Measurement of free radical products. 3.1: Lipids (i): peroxides and other products. 3.2: Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal. 3.3: Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipid peroxidation. 3.4: Proteins (i): determination of carbonyl groups in oxidized proteins. 3.5: Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA. 3.6: Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems. 3.7: Nucleic acids: measurement of products of free radical attack on nucleic acids. 4: Measurement of antioxidants. 4.1: Glutathione. 4.2: Glutathione peroxidase: activity and steady-state level of mRBA. 4.3: Superoxide dismutase. 4.4: Application of deuterium labelling and gas chromatography-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids. 4.5: Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants. 4.6: Measurement of antioxidant gene expression. Appendix