Free Radicals
A Practical Approach
Published on 22. August 1996
Book
Paperback/Softback
334 pages
978-0-19-963559-7 (ISBN)
Description
Free radicals - reactive, short-lived molecules - are now known to play an important role in a large number of human diseases, including heart disease and cancer, and their study in biological systems has increased enormously in recent years. This volume brings together practical techniques for detecting and measuring free radicals, and for studying their effects. It will be of interest to a wide cross-section of biochemists and clinicians.
Reviews / Votes
will greatly assist any laboratory wishing to enter the fray in what must be one of the most exciting areas of medicine, nutritional science and food technology ... the introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts for which the protocols are relevant. Thus this book will be not just a laboratory manual but should be a bestseller in the student market. * Times Higher Education Supplement *More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Illustrations
halftone and line figures, tables
Dimensions
Height: 235 mm
Width: 156 mm
Thickness: 18 mm
Weight
580 gr
ISBN-13
978-0-19-963559-7 (9780199635597)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Introduction ; 1. Direct and indirect measurements of free radicals by physico-chemical methods ; 1.1 In vitro and in vivo detection of free radicals metabolites with electron spin resonance ; 1.2 The application of high-field nuclear magnetic resonance spectroscopy to the detection of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs ; 2. Direct and indirect measurement of free radicals by other methods ; 2.1 Intrinsic (low-level) chemiluminescence ; 2.2 Visual assessment of oxidative stress by multifunctional digital microfluorography ; 2.3 Salicylic acid and phenylalanine as probes to detect hydroxyl radicals ; 3. Measurement of free radical products ; 3.1 Lipids (i): peroxides and other products ; 3.2 Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal ; 3.3 Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipid peroxidation ; 3.4 Proteins (i): determination of carbonyl groups in oxidized proteins ; 3.5 Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA ; 3.6 Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems ; 3.7 Nucleic acids: measurement of products of free radical attack on nucleic acids ; 4. Measurement of antioxidants ; 4.1 Glutathione ; 4.2 Glutathione peroxidase: activity and steady-state level of mRBA ; 4.3 Superoxide dismutase ; 4.4 Application of deuterium labelling and gas chromatography-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids ; 4.5 Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants ; 4.6 Measurement of antioxidant gene expression ; Appendix