Proteins LabFax
Published on 21. December 1995
Book
Hardback
318 pages
978-0-12-564710-6 (ISBN)
Description
Proteins Labfax is a detailed and comprehensive compendium of information on protein chemistry, and the purification and physical characterization of proteins. The book provides data on all the procedures usedon a regular basis by researchers studying proteins, including chromatography, crystallization, size determination, computer-aided structure analysis, amino acid analysis, and the chemical modification of amino acid side chains. General data is also givenon buffers, salting out, criteria of purity, expression systems, and protein stability.The contents of Protein Labfax have been carefully chosen to complement the data presented in its companion volume, Enzymology Labfax.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Ideal for biochemists, particularly enzymologists, molecular biologists, cell biologists, and researchers in life sciences in general.
Dimensions
Height: 241 mm
Width: 165 mm
Weight
860 gr
ISBN-13
978-0-12-564710-6 (9780125647106)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Purification:
Choice of Assay or Detection Method.
Preparation of Cell Extracts.
Buffers.
The Determination of Protein Concentration.
Salting Out: Ammonium Sulfate Precipitation.
Chromatographic Procedures.
Covalent Chromatography by Thiol-Disulfide Interchange using Solid-Phase Alkyl 2-Pyridyl Disulfide.
Criteria of Protein Purity.
Electrophoresis Methods.
Integral Membrane Proteins.
Expression Systems and Fusion Proteins.
Inclusion Bodies and Refolding.
Industrial Scale Purification of Proteins.
Peptide Synthesis.
Crystallization Procedures.
Physical Characterization:
Size Determination of Proteins: Hydrodynamic Methods and Mass Spectromic Methods.
FTIR and Raman Spectroscopies in the Study of Proteins and Other Biological Molecules.
Circular Dichroism.
NMR Structure Determination of Proteins.
EPR Spectroscopy of Proteins. Protein Stability.
Computer Analysis of Protein Structure.
Protein Chemistry:
Protein Chemistry Methods, Post-Translational Modification,Consensus Sequences.
Chemical Modification of Amino Acid Side-Chains.
Affinity-Based Covalent Modification.
Cross-Linking Reagents for Proteins.
Chapter References.
Subject Index.
Choice of Assay or Detection Method.
Preparation of Cell Extracts.
Buffers.
The Determination of Protein Concentration.
Salting Out: Ammonium Sulfate Precipitation.
Chromatographic Procedures.
Covalent Chromatography by Thiol-Disulfide Interchange using Solid-Phase Alkyl 2-Pyridyl Disulfide.
Criteria of Protein Purity.
Electrophoresis Methods.
Integral Membrane Proteins.
Expression Systems and Fusion Proteins.
Inclusion Bodies and Refolding.
Industrial Scale Purification of Proteins.
Peptide Synthesis.
Crystallization Procedures.
Physical Characterization:
Size Determination of Proteins: Hydrodynamic Methods and Mass Spectromic Methods.
FTIR and Raman Spectroscopies in the Study of Proteins and Other Biological Molecules.
Circular Dichroism.
NMR Structure Determination of Proteins.
EPR Spectroscopy of Proteins. Protein Stability.
Computer Analysis of Protein Structure.
Protein Chemistry:
Protein Chemistry Methods, Post-Translational Modification,Consensus Sequences.
Chemical Modification of Amino Acid Side-Chains.
Affinity-Based Covalent Modification.
Cross-Linking Reagents for Proteins.
Chapter References.
Subject Index.