G-pcr

1. Measurement of ligand-G protein-coupled receptor interactions using radioligand binding techniques Arthur Christopoulos, Katie Leach, Celine Valant, and Patrick Sexton, all at Department of Pharmacology, Monash University 2. Second messenger assays for G protein-coupled receptors; cAMP, Ca2+, inositol phosphates, ERK1/2 Patrick Sexton, Karen Greogry, Arthur Christopoulos and Caroline Hick, all at Department of Pharmacology, Monash University 3. Use of the [35S]GTPgS binding assay to determine ligand efficacy at G protein-coupled receptors Elodie Kara and Philip Strange, School of Pharmacy, University of Reading 4. Quantitative imaging of receptor trafficking Andy James and Anne Stephenson, School of Pharmacy, University of London; Takeo Awaji, Tokyo Women's Medical University School of Medicine; and Nicholas Hartell, Department of Cell Physiology and Pharmacology, University of Leicester 5. Production of recombinant GPCRs in yeast for structural and functional analysis Richard Darby, Mohammed Jamshad, Ljuban Grgic, William Holmes and Roslyn Bill, School of Life and Health Sciences, Aston University 6. Monitoring GPCR-protein complexes using BRET Werner Jaeger, Kevin Pfleger and Karin Eidne , all at QEII Medical Centre, Perth 7. Using intra-molecular FRET to study receptor conformation Cornelius Krasel, School of Pharmacy, University of Reading, and Carsten Hoffmann, Department of Pharmacology, University of Wuerzburg 8. A disulfide cross-linking strategy useful for studying ligand-induced structural changes in GPCRs Jian Hua Li, Stuart D.C. Ward, Sung-Jun Han, Fadi F. Hamdan, and Jurgen Wess, all at the National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 9. Use of fluorescence correlation spectroscopy to study the diffusion of GPCRs Stephen J. Briddon, Jonathan A. Hern and Stephen J. Hill, all at the Institute of Cell Signalling, School of Biomedical Sciences, University of Nottingham 10. Identification and proteomic analysis of GPCR phosphorylationKok Choi Kong, Sharad C. Mistry and Andrew B. Tobin, all at Department of Cell Physiology and Pharmacology, University of Leicester 11. Measurement and visualisation of G protein-coupled receptor trafficking by ELISA and immunofluorescence Stuart J Mundell, Shaista P Nisar and Eamonn Kelly, all at Department of Pharmacology, University of Bristol 12. Substituted cysteine accessibility method (SCAM) George Liapakis, Faculty of Medicine, University of Crete; and Jonathan Javitch, Center for Molecular Recognition, Columbia University 13. Homology modeling of GPCRs John Simms, Department of Pharmacology, Monash University Appendix. Site-directed mutagenesis and chimeras Alex Conner, School of Medicine, University of Warwick; David Poyner, School of Medicine, Aston University; and Mark Wheatley, School of Medicine, Birmingham University List of suppliers Index