New Frontiers in the Study of Gene Functions
Published on 14. October 2011
Book
Paperback/Softback
218 pages
978-1-4612-9030-8 (ISBN)
Description
The heat-shock proteins in E. coli are transiently overexpressed af- ter shift to a higher growth temperature. The genes that encode the HSPs are preceded by promoters transcribed in vitro by a form of RNA poly- 32 32 merase holoenzyme containing a 32-kd a subunit (Ea ). The a subunit is encoded by the rpoH (htpR) gene, previously identified as a positive 32 effector of the heat-shock response. Our evidence suggests that Ea is the enzyme that transcribes heat-shock genes at all temperatures. The level 32 of a may be regulated at several points: Accumulation of rpoH mRNA 32 is affected by temperature shift, a synthesis is regulated posttranscrip- 32 tionally, and a is an unstable molecule with a tl/2 of 5 min. Many mu- tations in the HSPs are shown to have defects in proteolysis. References Baker. T. A. , Grossman. A. D . . and Gross. C. A. , 1984, A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. A cad. Sci. US. A. 81:6779-6783. Bardwell, J. C. A . . and Craig, E. A . . 1984. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous, Proc. Natl. Acad. Sci. US. A. 81:848-852. Bukhari. A. I. .
and Zipser. D . . 1973, Mutants of Escherichia coli with a defect in the degradation of nonsense fragments, Nature New Bioi. 243:238-241. Charette. M. F. , Henderson, G. W. , and Markovitz, A.
and Zipser. D . . 1973, Mutants of Escherichia coli with a defect in the degradation of nonsense fragments, Nature New Bioi. 243:238-241. Charette. M. F. , Henderson, G. W. , and Markovitz, A.
More details
Series
Edition
Softcover reprint of the original 1st ed. 1987
Language
English
Place of publication
New York
United States
Target group
Professional and scholarly
Research
Illustrations
27 s/w Abbildungen
218 p. 27 illus.
Dimensions
Height: 229 mm
Width: 152 mm
Thickness: 13 mm
Weight
326 gr
ISBN-13
978-1-4612-9030-8 (9781461290308)
DOI
10.1007/978-1-4613-1845-3
Schweitzer Classification
Other editions
Additional editions
George Poste | Stanley T. Crooke
New Frontiers in the Study of Gene Functions
Book
01/1987
Kluwer Academic / Plenum Publishers
€85.59
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Content
1 The Role of Cis- and Trans-Acting Functions in Simian Virus 40 Gene Regulation.- 1. Introduction.- 2. Early Viral Transcriptional Program.- 3. Late Viral Transcriptional Program.- 4. Discussion.- References.- 2 Regulation of the Heat-Shock Response in Escherichia coli.- 1. Introduction.- 2. Transcription of the Heat-Shock Genes.- 3. Regulation of rpoH and ?32.- 4. Function of the Heat-Shock Proteins.- 5. Summary.- References.- 3 Negative Control of DNA Replication Revealed in Composite Simian Virus 40-Bovine Papillomavirus Plasmids.- 1. Overview.- 2. Introduction.- 3. Results.- 4. Discussion.- References.- 4 DNA Supercoiling as a Regulator of Bacterial Gene Expression.- 1. Introduction.- 2. Results and Discussion.- References.- 5 Retrotransposition in Yeast.- 1. Introduction.- 2. Experiments.- 3. Speculation.- References.- 6 Comparative Genetic Analysis of Homeobox Genes in Mouse and Man.- 1. Introduction.- 2. Murine Homeobox Genes.- 3. Human Homeobox Genes.- 4. Comparative Genetic Relationships between Species.- 5. Comparative Genetic Relationships within Species.- 6. Possible Functional Relationships between Homeobox Loci and Linked Genes.- 7. Discussion.- References.- 7 Eukaryotic Transcriptional Specificity Conferred by DNA-Binding Proteins.- 1. Introduction.- 2. Results and Discussion.- 3. Concluding Remarks.- References.- 8 Chromatin Structure Near an Expressed Gene.- 1. Introduction.- 2. Results.- 3. Discussion.- References.- 9 Specificity of Gene Expression and Insertional Mutagenesis in Transgenic Mice.- 1. Introduction.- 2. Experiments and Results.- References.- 10 Retroviruses as Insertional Mutagens.- 1. Introduction.- 2. Insertion of Moloney Murine Leukemia Viruses into the Germ Line of Mice.- 3. Expression of the Proviral Genome in Mov Substrains ofMice.- 4. Induction of Two Insertional Mutations.- 5. Insertional Mutagenesis by Retroviruses and DNA Injection.- References.- 11 P Transposable Elements and Their Use as Vectors for Gene Transfer in Drosophila.- 1. Introduction.- 2. Results and Discussion.- References.- 12 Mapping and Manipulating Immunoglobulin Functions.- 1. Introduction.- 2. Immunoglobulin Gene and Protein Structure.- 3. Production of Monoclonal Immunoglobulins from Mouse and Man.- 4. Uses of Specific Immunoglobulins.- 5. Optimizing Immunoglobulin Structure for Therapy.- 6. Identifying the Molecular Requirements for Immunoglobulin Synthesis and Function.- 7. Future Studies on the Structural and Regulatory Mutants.- References.- 13 T4: A T-Cell Surface Protein Mediating Cell-Cell and Cell-AIDS Virus Interactions.- 1. Introduction.- 2. Results.- References.- 14 Identifying the Determinants of Protein Function and Stability.- 1. Introduction.- 2. Repressor and Cro Background.- 3. Isolation of Phenotypically Defective Mutants.- 4. Structural Distribution of Mutant Sites.- 5. Why Are Mutant Proteins Nonfunctional?.- 6. Phenotypic Reversion.- 7. Summary.- References.