RNA Editing
Description
This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Content
1. Substitutional RNA Editing in Plant Organelles
Mizuho Ichinos and Mamoru Sugita
2. Computational Detection of Plant RNA Editing Events
Alejandro A. Edera and M. Virginia Sanchez-Puerta
3. Discovering RNA Editing Events in Fungi
Huiquan Liu and Jin-Rong Xu
4. C-to-U RNA Editing: From Computational Detection to Experimental Validation
Taga Lerner, Mitchell Kluesner, Rafail Nikolaos Tasakis, Branden S. Moriarity, F.N. Papavasiliou, and Riccardo Pecori
5. Live Cell Quantification of APOBEC1-mediated RNA Editing: A Comparison of RNA Editing Assays
Martina Chieca, Serena Torrini, and Silvestro G. Conticello
6. Adenosine to Inosine RNA Editing Enzyme ADAR and microRNAs
Kang Yuting, Dan Ding, and Hisashi Iizasa
7. Quantitative Analysis of Adenosine-to-Inosine RNA Editing
Turnee N. Malik, Jean-Philippe Cartailler, and Ronald B. Emeson
8. Discovering A-to-I RNA Editing Through Chemical Methods
Masayuki Sakurai, Shunpei Okada, Hiroki Ueda, and Yang Yuxi
9. ALU A-to-I RNA Editing - Millions of Sites and Many Open Questions
Amos Schaffer and Erez Y. Levano
10. RNA Editing in Human and Mouse Tissues
Harini Srinivasan, Eng Piew Louis Kok, and Meng How Tan
11. Bioinformatics Resources for RNA Editing
Maria Angela Diroma, Loredana Ciaccia, Graziano Pesole, and Ernesto Picardi
12. High-throughput Sequencing to Detect DNA-RNA ChangesClaudio Lo Giudice, Graziano Pesole, and Ernesto Picardi
13. Detection of A-to-I Hyper-Edited RNA Sequences
Roni Cohen-Fultheim and Erez Y. Levanon
14. Proteome Diversification by RNA Editing
Eli Eisenberg
15. MicroRNA Editing Detection and Function: A Combined in silico and Experimental Approach for the Identification and Validation of Putative Oncogenic Targets
Valentina Tassinari, Valeriana Cesarini, Domenico Alessandro Silvestris, Andrea Scafidi, Lorenzo Cucina, and Angela Gallo
16. RNA Editing in Interferonopathies
Loredana Frassinelli, Silvia Galardi, Silvia Anna Ciafrè, and Alessandro Michienzi
17. The Role of RNA Editing in the Immune Response
Sadeem Ahmad, Xin Mu, and Sun Hur
18. RNA Editing in Neurological and Neurodegenerative Disorders
Pedro Henrique Costa Cruz and Yukio Kawahara
19. New Frontiers for Site-directed RNA Editing - Harnessing Endogenous ADARs
Tobias Merkle and Thorsten Stafforst