Methods and Techniques in Virology

Laboratory Safety Monolayer Cell Culture Isolation and Identification of Viruses Animal Models Inoculation of Laboratory Animals Concentration of Viruses Protein Assays Ultracentrifugation Chromatography Electrophoresis Electron Microscopy Serology Immunofluorescence Immunoperoxidase ELISA Monoclonal Antibodies: Identification of Antigenic Sites Histology and Histochemistry Immunocytochemical Staining Electron Microscopy Metabolic and Chemical Radiolabeling Identification of Viral Proteins by Immunoprecipitation Identification of Viral Proteins by Western Blotting Peptide Mapping Agarose Gel Electrophoresis of DNA Molecules Extraction of Viral Nucleic Acids In Vitro Translation Cloning of Genes from DNA Viruses Synthesis of Complementary DNA Spreading and Visualization of Nucleic Acids by Electron Microscopy Hybridization of Nucleic Acids Polymerase Chain Reaction