Genome Editing in Biomedical Sciences
Description
This volume focuses on applying the Crispr system in editing the genome of human cells ( in vitro and in vivo ) and model organisms used in biomedical research. With the advent of Crispr technology, genome editing soon became a procedure of great interest to laboratories worldwide due to its relative ease and accuracy. In biomedical sciences, genome editing by Crispr has already enabled the development of new experimental model systems. In medicine, therapeutic alternatives for the genetic "correction" of diseases have already begun to appear. Therefore, the book's purpose is to bring in a single volume, chapters that show the scientific community in biomedicine, medicine, human genetics, oncology, virology, and parasitology, among others, the advances in genomic editing. In a chapter dedicated to the ethical aspects of human genomic editing, we also address what we can and should do with this (bio)technology. The book chapters were written by productive researchers specializing in Crispr genome editing. The chapters cover the concept of Crispr and genome editing and how to use this new methodology in biomedical research and medicine, among other aspects, including the ethical controversy around its use in humans. The writing of the chapters keeps a specialized language intelligible enough for those who want to introduce themselves to the subject.
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Geraldo A. Passos received his Ph.D. degree in biochemistry in 1988 from Ribeirão Preto Medical School, University of São Paulo (USP), Brazil. His postdoctoral studies were conducted at the Molecular Genetics Institute of Montpellier (Centre National de la Recherche Scientifique, CNRS), France (1992-1994), where he worked with sequencing and physical mapping of the human immunoglobulin lambda locus on chromosome 22q11.2. For several years, he worked in close collaboration with the transcriptome and microarray team of the TAGC Lab (Technologies Avancées pour le Génome et la Clinique) at the Centre d´Immunologie de Marseille-Luminy in Marseille, France (1999-2001), and then at the Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1090 at the Luminy Scientific Park in Marseille, France (2002-2017) to study the large scale gene expression profiling of the murine thymus gland. He is currently an Associate Professor of Genetics and Molecular Biology at the School of Dentistry of Ribeirão Preto, USP, and a collaborator at the Ribeirão Preto Medical School, USP, where he is the head of the Molecular Immunogenetics Group.
Content
What is the CRISPR system and how it is used?.- Modern approaches to mouse genome editing using the CRISPR-Cas toolbox and their applications in functional genomics and translational research.- Long Non-Coding RNAs and Crispr-Cas edition in Tumorigenesis.- The Crispr/Cas system in human cancer.- Using CRISPR/Cas9 to Edit a Thyroid Cancer Cell Line.- Genome editing for engineering the next generation of advanced immune cell therapies.- CRISPR Genome Editing and the Study of Chagas Disease.- Genome Editing Tools for Lysosomal Storage Disorders.- CRISPR Libraries and Whole-Genome Screening to Identify Factors for Viral Infections.- Gene Editing Technologies Targeting TFAM and its Relation to Mitochondrial Diseases.- The Gene-Edited Babies Controversy: Reactions in the Scientific Community, Social Media, and the Press.- Index.