Flow Cytometry
Ormerod(Author)
Oxford University Press
3rd Edition
Published on 1. September 2000
Book
Hardback
296 pages
978-0-19-963825-3 (ISBN)
Description
This text is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. The text aims to cover over 90 per cent of all applications of flow cytometry in mammalian biology.
More details
Edition
3rd Revised edition
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Edition type
Revised edition
Illustrations
figures
ISBN-13
978-0-19-963825-3 (9780199638253)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Other editions
Previous edition
M.G. Ormerod
Flow Cytometry
Book
06/1994
2nd Edition
Oxford University Press
€37.08
Article exhausted; check for reprint
Content
Introduction to the principles of flow cytometry; fluorescence and fluorescence technology; preparing of suspensions of single cells; flow sorting; immunofluorescence of surface markers; analysis of DNA - general methods; further clinical applications; quality control in the clinical laboratory; measurement of cytoplasmic and nuclear antigens; analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method; analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method; chromosome analysis and sorting by flow cytometry; intracellular ionised calcium, magnesium, membrane potential and pH; flow cytometry in the study of apoptosis; further applications to cell and molecular biology.