Baculovirus Expression Vectors
A Laboratory Manual
Published on 12. May 1994
Book
Spiral bound
368 pages
978-0-19-509131-1 (ISBN)
Description
This laboratory manual simplifies selection of the most appropriate baculovirus vector design for a given problem. It then describes each step of the implementation process - from vector construction to large-scale protein production. The book provides an understanding of how the vectors work, a biological overview of cells, viruses, plasmids, and promoters, guidelines for choosing optimum vectors, protocols for growing insect cells and recombinant viruses, methods for analysing protein products and scaling up protein production, techniques for producing proteins in insect larvae, and easy to use maps charting available expression vectors.
Reviews / Votes
"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, etal. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell
"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview
prior to taking the plunge." --Tibtech
"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et
al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell
"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview
prior to taking the plunge." --Tibtech
More details
Language
English
Place of publication
New York
United States
Target group
Professional and scholarly
Illustrations
halftones, line figures, tables
Dimensions
Height: 235 mm
Width: 279 mm
Thickness: 23 mm
Weight
774 gr
ISBN-13
978-0-19-509131-1 (9780195091311)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Acknowledgments How To Use This Manual PART I: AN OVERVIEW OF BACULOVIRUSES 1: Virus Structure and the Infection Process 2: Gene Organization, Regulation, and Function 3: Virus-Host Interactions 4: Summary of Baculovirus Features Relevant to Expression Factors PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS 5: Choice of Virus and Host Species 6: Choice of Transfer Plasmid 7: Available Transfer Plasmids 8: Choosing a Parent Virus for Use in Vector Construction 9: Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION 10: Safety Considerations 11: Insect Cell Culture 12: Virus Methods 13: Contransfection and Recombinant Virus Identification 14: Characterizing Recombinant Gene Expression 15: Post-Translational Modification PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE 16: David L. Clemm: Scale-Up of Protein Production in a Stirred Bioreactor 17: Cheryl Isaac Murphy: Scale-Up of Protein Production in an Airlift Fermenter 18: Insect Rearing and Infection Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region Appendix 2: Nucleotide Sequence of AcMNPV p10 Region Appendix 3: List of Genes Expressed in the Baculovirus Expression System Appendix 4: Suppliers Appendix 5: Stock Solutions Appendix 6: Excel Spreadsheet for TCID[5[0 Calculation References Index