Antibody Phage Display
Methods and Protocols
Published on 28. December 2001
Book
Paperback/Softback
XV, 401 pages
978-0-89603-711-3 (ISBN)
Description
The closing years of the 19th century and the start of the 20th century witnessed the emergence of microbiology and immunology as discrete sci- tific disciplines, and in the work of Roux and Yersin, perhaps the first benefits of their synergy-immunotherapy against bacterial infection. As we advance into the new millennium, microbiology and immunology again offer a c- ceptual leap forward as antibody phage display gains increasing acceptance as the definitive technology for monoclonal production and unleashes new - portunities in immunotherapy, drug discovery, and functional genomics. In assembling Antibody Phage Display: Methods and Protocols, we have aimed to produce a resource of real value for scientists who have followed the development of phage display technology over the past decade. The founding principles of phage display have always held an elegant simplicity. We hope that readers will find similar clarity in the technical guidance offered by the book's contributors. In meeting our objectives, we have tried to cover the broad scope of the technology and the key areas of library construction, scre- ing, antibody modification, and expression. Of course, the technology cont- ues to advance apace, but we trust that readers will be able to gage the potential of phage display from our coverage, that some of its subtleties will emerge, and that our selection of methods will prove appealing. We are indebted to all the contributing authors for sharing their expertise with the wider scientific community.
Reviews / Votes
"...this book lives up to its promises. It starts with an excellent introduction by Hennie Hoogenboom, reprinted from his Immunotechnology article, which sets the scene and provides an overview of the field for new readers...Members of the laboratory that used the book for reference found it very clear and all were keen that the review was completed as quickly as possible so that the book was returned to the laboratory where they could make use of it! I think this in itself supports the fact that the book is a useful volume." - Immunology News"As with other volumes in this popular series, each protocol is discussed in detail, including an introduction, materials, methods notes, and references. It is a good purchase for research libraries where monoclonal antibodies are studied." E-Streams
More details
Series
Edition
2002 ed.
Language
English
Place of publication
Totowa
United States
Target group
Professional and scholarly
Research
Illustrations
XV, 401 p.
Dimensions
Height: 23.5 cm
Width: 15.5 cm
Weight
617 gr
ISBN-13
978-0-89603-711-3 (9780896037113)
DOI
10.1385/1592592406
Schweitzer Classification
Other editions
New editions
Book
07/2009
2nd Edition
Humana Press Inc.
€160.49
Shipment within 15-20 days
Additional editions
Book
12/2009
Humana Press Inc.
€171.19
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Content
Overview of Antibody Phage-Display Technology and Its Applications.- Standard Protocols for the Construction of Fab Libraries.- Standard Protocols for the Construction of scFv Libraries.- Broadening the Impact of Antibody Phage Display Technology.- Construction of Large Naïve Fab Libraries.- Construction of Polyclonal Antibody Libraries Using Phage Display.- Antigen-Driven Stimulation of B-Lymphocytes In Vitro.- 8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion.- Panning of Antibody Phage-Display Libraries.- Selection of Antibodies Against Biotinylated Antigens.- Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries.- Blocking Immunodominant Epitopes by Competitive Deselection.- Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy.- Screening of Phage-Expressed Antibody Libraries by Capture Lift.- Antibody-Guided Selection Using Capture-Sandwich ELISA.- Proximity-Guided (ProxiMol) Antibody Selection.- Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies.- Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques.- Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs.- Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments.- Selection of Antibodies Based on Antibody Kinetic Binding Properties.- Selection of Functional Antibodies on the Basis of Valency.- Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis.- Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement.- Error-Prone Polymerase Chain Reaction for Modification of scFvs.- Use of Escherichia coli Mutator Cells to Mature Antibodies.- ChainShuffling to Modify Properties of Recombinant Immunoglobulins.- Generation of Bispecific and Tandem Diabodies.- High-Level Periplasmic Expression and Purification of scFvs.- Periplasmic Expression and Purification of Recombinant Fabs.- Expression of Antibody Fragments in Pichia pastoris.- Expression of VHH Antibody Fragments in Saccharomyces cerevisiae.- Intrabodies.- 34 Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells.- 35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells.