PCR

Background to PCR and Its Application Areas (C. Newton). Setting Up a PCR Laboratory (C. Newton). Instruments (C. Newton). Nucleic Acid Substrates (C. Newton). Thermostable DNA Polymerases (C. Newton). Primers (C. Newton). The Synthesis of Chemically Labeled PCR Primers (T. Brown & D. Brown). Protocol Optimization and Reaction Specificity (S. Powell). Contamination Avoidance (C. Newton). Use of 5' Chemically Labeled Primers in PCR (J. Grzybowski, et al.). Detection of PCR Products (L. MacCallum). Purification of PCR Products (C. Newton). Cloning PCR Products (C. Newton). Miscellaneous Data (C. Newton). Troubleshooting (C. Newton). Manufacturers and Suppliers (C. Newton). References. Further Reading. Appendix. Index.