PCR
C.R. Newton(Editor)
Wiley (Publisher)
Published on 26. June 1995
Book
Paperback/Softback
236 pages
978-0-471-95222-0 (ISBN)
Description
This handy affordable lab reference allows quick access to fundamental techniques needed to undertake PCR. Topics covered include: background to PCR and its application; laboratory set--up; instruments; protocols; primers; detection and purification; methods for cloning PCR products; troubleshooting tips and more.
More details
Series
Language
English
Place of publication
Chichester
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Illustrations
Illustrations
Dimensions
Height: 190 mm
Width: 110 mm
Weight
250 gr
ISBN-13
978-0-471-95222-0 (9780471952220)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
Background to PCR and Its Application Areas (C. Newton). Setting Up a PCR Laboratory (C. Newton). Instruments (C. Newton). Nucleic Acid Substrates (C. Newton). Thermostable DNA Polymerases (C. Newton). Primers (C. Newton). The Synthesis of Chemically Labeled PCR Primers (T. Brown & D. Brown). Protocol Optimization and Reaction Specificity (S. Powell). Contamination Avoidance (C. Newton). Use of 5' Chemically Labeled Primers in PCR (J. Grzybowski, et al.). Detection of PCR Products (L. MacCallum). Purification of PCR Products (C. Newton). Cloning PCR Products (C. Newton). Miscellaneous Data (C. Newton). Troubleshooting (C. Newton). Manufacturers and Suppliers (C. Newton). References. Further Reading. Appendix. Index.