Culture Techniques

Frontmatter -- Dedication -- Preface -- Table of Contents -- Introduction / Neubert, D. -- I. Studies on Whole-Embryo Culture -- Differentiation of Embryonic Tissues in Whole-Embryo Cultures as Compared to the Development In Vivo / Herken, R. / Anschütz, M. -- The Differentiation Between Teratogenic Action on Mother, Fetal Membranes and Embryos Using the Whole-Embryo Culture Method / Beck, F. -- Trypan Blue Teratogenesis in the Rat In Vitro / Gulamhusein, A. P. -- Studies on Embryotoxic Effects of Thallium Using the Whole-Embryo Culture Technique / Anschütz, M. / Herken, R. / Neubert, D. -- Evaluation of Serum Teratogenic Activity Using Rat Embryo Cultures / Klein, N. W. / Plenefisch, J. D. / Carey, S. W. / Chatot, C. L. / Clapper, M. L. -- Regulation of Mouse Embryogenesis by Exogenous Growth Factors / Hsu, Y. -C. -- Preliminary Communication on the Feasibility of Culturing Whole Embryos in a Chemically Defined Medium / Klee-Trieschmann, U. / Neubert, D. -- In Vitro Development of the Heart Under Influence of Retinole Acid / Davis, L. A. / Sadler, T. W. / Langman, J. -- II. Studies on Organ Cultures -- A. Differentiation of the Skeleton (predominantly Extremities) -- Simulation of Limb Bud Skeletogenesis In Vitro / Merker, H. -J. / Krügerand, U. / Zimmermann, B. -- Comparison of the Differentiation of Muscle and Connective Tissue of Mouse Limb Buds in Culture and In Vivo: A Morphological Study by Indirect Immunofluorescence / Barrach, H. -J. / Grundmann, K. / Hinz, N. / Felies, A. -- Quantification of Collagen Types I and II in Mouse Limbs during Differentiation In Vitro and In Vivo / Dusemund, B. / Barrach, H. -J. -- Feasibility of Storing Embryonic Tissues for Subsequent Use in Organ Culture / Neubert, D. / Bluth, U. -- Limb Bud Organ Cultures from Mouse Embryos after Apparent Induction of Monooxygenases In Utero. Effects of Cyclophosphamide, Dimethylnitrosamine and Some Thalidomide Derivatives / Neubert, D. / Bluth, U. -- On the Significance of Ascorbate and of Cysteine on Differentiation of Limb Buds in Organ Culture / Blankenburg, G. / Bluth, U. / Neubert, D. -- Effects of Some "Indirectly" Alkylating Agents on Differentiation of Limb Buds in Organ Culture / Stahlmann, R. / Bluth, U. / Neubert, D. -- Comparison of Effects on Limb Development In Vivo and In Vitro Using Methyl(acetoxymethyl)nitrosamine / Bochert, G. / Platzek, T. / Wiessler, M. -- Development of Limb Buds in Organ Culture: Examination of Hydroxyurea Enhancement of Bromodeoxyuridine Toxicity Using Image Analysis / Kwasigroch, T. E. / Skalko, R. G. / Church, J. K. -- Biochemical Characterization of Mouse Hereditary Chondrodystrophies in Organ Culture / Brown, K. S. / Barrach, H.-J. / Cranley, R. E. / Greene, R. / Kimata, K. / Kleinman, H. K. / Pennypacker, J. P. -- Effect of Teratogens on Human Embryonic Skeletal Tissue In Vitro / Rajan, K. T. / Evans, P. H. / Merker, H.-J. -- Aspirin Induced Polydactyly in Rats Mediated by Inhibition of Prostaglandin Synthesis / Scott, W. J. / Klein, K. L. -- B. Differentiation of other Organ Anlagen (kidneys, lungs, heart, prostate gland, haematopoetic system, and intestine) -- The Developing Kidney as a Model System for Normal and Impaired Organogenesis / Saxen, L. / Ekblom, P. -- The Embryonic Lung as In Vitro Model for Testing Teratogenic Substances / Merker, H.-J. / Zimmermann, B. / Riso, J. -- The Toxic Effects of Metal Dusts on Human Foetal Lungs In Vitro / Evans, P. H. / Rajan, K. T. / Webb, M. -- Effects of Substances Influencing Glycosaminoglycan Synthesis on Lung Development in Culture / Riso, J. / Zimmermann, B. -- Effects of L-azetidine-2-carboxylic Acid and ß-D-xylosides on Lung Development In Vitro / Zimmermann, B. / Riso, J. / Somogyi, H. -- Interaction of Epithelium and Mesenchyme in the Induction of Foetal Rat and Mouse Prostate Glands by Androgens in Organ Culture / Lasnitzki, I. / Mizuno, T. -- In Vivo and In Vitro Assays of Trisomie Cells Isolated from the Fetal Organism or Rescued by Transfer to Non-Trisomic Hosts / Gropp, A. / Herbst, E. W. / Nielsén, K. -- Effects of Gliadin-derived Peptides from Bread and Durum Wheat on Small Intestine Cultures from Rat Fetus and Coeliac Children / Silano, V. / Auricchio, S. / De Ritis, G. / De Vincenzi, M. / Occorsio, P. -- III. Studies on Preimplantation Embryos -- Differential Adhesiveness as a Mechanism of Cell Allocation to Inner Cell.Mass and Trophectoderm in the Mouse Blastocyst / Surani, M. A. H. / Kimber, S. J. / Barton, S. C. -- Inefficient Capacity for Oxidative Phosphorylation of the Trophoblast as a Cause of Delayed Implantation in the Mouse / Nilsson, B. O. / Magnusson, C. -- Influence of Micromanipulation of Ova on the Postnatal Development of Mice (AKR) / Baunack, E. / Gärtner, K. -- Investigations on the Mechanism of Action and on the Pharmacokinetics of Cyclophosphamide Treatment during the Preimplantation Period in the Mouse / Spielmann, H. / Habenicht, U. / Eibs, H.-G. / Jacob-Müller, U. / Schimmel, A. -- Benzo(a)pyrene Metabolism in Early Mouse Embryos / Pedersen, R. A. -- IV. Studies on Cell and Tissue Cultures -- Influence of Different Antisera on Mouse Epiphyseal Chondrocytes in Monolayer Culture / Grundmann, K. / Zimmermann, B. / Barrach, H.-J. / Merker, H.-J. -- Cell Movement during Blastema Formation. Effect of Cytochalasin B on Cartilage Development In Vitro / Zimmermann, B. -- Cell-Substrate Adhesion of Fibroblast-like Cells Under Different In Vitro Conditions. A Scanning and Transmission Electron-Microscopic Study / Scharlach, E. / Zimmermann, B. -- Development of Cytochrome P-450-Dependent Drug Metabolizing Enzyme Activities in Mouse and Human Tissues In Vitro / Nau, H. / Gansau, C. -- In Vitro Treatment with Metallocene Dichlorides: Determination of the Intracellular Distribution of the Metal Atoms by Use of the Electron Energy Loss Spectroscopy / Köpf-Maier, P. / Krahl, D. -- Morphological Changes Induced by 2,3,7,8-Tetrachlorodibenzo- p-dioxin (TCDD) in Epithelial Monolayer Culture Using Human and Rodent Fetal Tissues / Nau, H. / Merker, H.-J. / Richter, H. -- V. Studies on Non-mammalian Tissues -- The Chick Embryo: A Standard Against Which to Judge In Vitro Systems / Summerbeir, D. / Hornbruch, A. -- Experimental Manipulation Leading to Cardiac Malformation in Chick Embryo / Steding, G. / Seidl, W. / Sydow, H. / Bahners, W. / Borowsky, D. -- Morphogenese Systems and In Vitro Techniques in Teratology / Jelinek, R. / Peterka, M. -- VI. Culture Techniques for "Screening" on Teratogenicity -- An Assessment of the Available In Vitro Techniques for Detecting Teratogens / Flint, O. P. -- On the Predictability of Developmental Toxicity -especially Prenatal Toxicity - On the Basis of Culture Experiments / Neubert, D. -- VII. Some Methods Used for Culturing Embryonic Tissues -- A. Whole-Embryo-Culture: Beck; Anschiitz -- B. Limb Bud Method: Blankenburg -- C. Trowell Method: Zimmermann -- D. "Micro-Mass Culture": Zimmermann -- E. Preimplantation-Embryo Culture -- F. Teratogenicity Testing Method (Chick) -- G. CHEST I and II (Chick): Jelinek and Peterka -- Glossary -- List of Participants -- Index -- Backmatter