Gene Therapy Protocols

Name: Gene Therapy Protocols
Brand: Humana Press Inc.
Price: 85.59 EUR
Availability: OutOfStock

Jeffrey R. Morgan(Editor)

Humana Press Inc.

2nd Edition

Published on 10. October 2001

Book

Paperback/Softback

XV, 520 pages

978-0-89603-869-1 (ISBN)

€85.59incl. 7% vat

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Description

Efforts in gene therapy have grown dramatically in recent years. Basic research as well as clinical activity have made exciting progress and are beg- ning to offer renewed hope that gene therapy may be able to deliver novel approaches for the treatment of inherited as well as such acquired diseases as cardiovascular disease and cancer. With the sequencing of the human genome complete, we now have a comprehensive catalog of genes that further expands the potential role of gene therapy into such new fields as tissue engineering. Central to gene therapy is the process of gene transfer; thus, advances in the technology of gene transfer are at the heart of this field's progress. Numerous technologies, based on a variety of methods (e.g., viral-mediated, physical/ chemical), have been developed to achieve gene transfer. Some of the earliest methods, such as recombinant retroviruses, are still widely used, have undergone significant improvements, and have given rise to new vectors based on lentiviruses.

Reviews / Votes

From Reviews of the First Edition:
"...extremely impressive...a must, even for researchers who are already familiar with gene transfer techniques..." -Molecular Medicine Today

"...succeeds admirably..."-Genetical Research

From Reviews of the Second Edition:

"Each protocol is written in a standard format, consisting of introduction, materials, methods, notes, and references. The introductions vary in length and detail. Many of them are illustrated with tables, charts, schematics, and/or photographs. The materials and methods sections are quite detailed, and there are many notes clarifying steps or outlining potential problems. Gene Therapy Protocols is recommended for university, hospital, and corporate libraries serving researchers who are working on gene therapies." - E-STREAMS

"All the chapters are well written, and the protocols are easy to understand. This book is applicable to those directly involved in the field of gene therapy." - Pharmaceutical Research

"...a valuable resource for background information in the field of gene transfer vector development, as well as providing concise and clearly written protocols for the production of these vectors." - Molecular Biotechnology

"Each of the 32 chapters is constructed like a cooking-book with detailed instructions and illustrations of how to perform the procedures. For anyone who is planning or is already practically involved in gene therapy experiments, this impressive book is a must in the laboratory." - Acta Derm Venereol

"Second edition of the "Gene Therapy Protocols" in successful series of books "Methods in Molecular Medicine" from Humana Press, is certainly a publication, which appeared in the right time. The comprehensibility of the book represents great value also for researcher. This collection of gene therapy protocols is a valuable and indispensable resource for basic scientists, graduate students, and postdoctoral fellows. Even clinical researchers will find the book very informative and useful. The book represents an outstanding work, which should not be missed in all laboratories dealing with gene therapy." - Neoplasma

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Content

Poly-l-Lysine-Based Gene Delivery Systems: Synthesis, Purification, and Application Charles P. Lollo, Mariusz G. Banaszczyk, Patricia M. Mullen, Christopher C. Coffin, Dongpei Wu, Alison T. Carlo, Donna L. Bassett, Erin K. Gouveia, and Dennis J. Carlo Targeted Gene Transfer to Liver Using Protein-DNA Complexes Catherine H. Wu, Cherie M. Walton, and George Y. Wu Receptor-Directed Molecular Conjugates for Gene Transfer Assem G. Ziady and Pamela B. Davis Gene Transfer into Muscle by Electroporation in Vivo Jun-ichi Miyazaki and Hiroyuki Aihara Viral Liposomes: Preparation and Use Yasufumi Kaneda LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery Yadi Tan, Mark Whitmore, Song Li, Peter Frederik, and Leaf Huang Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use Klaus Schughart and Ulla B. Rasmussen Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts Olga A. Igoucheva and Kyonggeun Yoon Regulated Expression of Plasmid-Based Gene Therapies Ronald V. Abruzzese, Fiona C. MacLaughlin, Louis C. Smith, and Jeffrey L. Nordstrom Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery Lina Prasmickaite, Anders Høgset, and Kristian Berg Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun Chia-Feng Kuo, Jeng Hwan Wang, and Ning-Sun Yang Preparation of Pseudotyped Retroviral Vector Hong Yu Quantitative Measurement of the Concentration of Active Recombinant Retrovirus Stelios T. Andreadis and Jeffrey R. Morgan Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use Jarmo Wahlfors and Richard A. Morgan Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells William P. Swaney,Enrico M. Novelli, Alfred B. Bahnson, and John A. Barranger Genetically Modified Skin Substitutes: Preparation and Use Karen E. Hamoen, Gulsun Erdag, Jennifer L. Cusick, Hinne A. Rakhorst, and Jeffrey R. Morgan Bioartificial Muscles in Gene Therapy Courtney Powell, Janet Shansky, Michael Del Tatto, and Herman H. Vandenburgh Cytokine Gene-Modified Cell-Based Cancer Vaccines R. Todd Reilly, Jean-Pascal H. Machiels, Leisha A. Emens, and Elizabeth M. Jaffee HIV-Based Vectors: Preparation and Use Antonia Follenzi and Luigi Naldini Packaging Cell System for Lentivirus Vectors: Preparation and Use Narasimhachar Srinivasakumar Lentiviral Vectors: Preparation and Use Lung-Ji Chang and Anne-Kathrin Zaiss Simian Foamy Virus Vectors: Preparation and Use Jeonghae Park and Ayalew Mergia Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use Michael A. Curran a

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