Gene Therapy Protocols
2nd Edition
Published on 15. October 2001
Book
Hardback
XV, 520 pages
978-0-89603-723-6 (ISBN)
Description
Efforts in gene therapy have grown dramatically in recent years. Basic research as well as clinical activity have made exciting progress and are beg- ning to offer renewed hope that gene therapy may be able to deliver novel approaches for the treatment of inherited as well as such acquired diseases as cardiovascular disease and cancer. With the sequencing of the human genome complete, we now have a comprehensive catalog of genes that further expands the potential role of gene therapy into such new fields as tissue engineering. Central to gene therapy is the process of gene transfer; thus, advances in the technology of gene transfer are at the heart of this field's progress. Numerous technologies, based on a variety of methods (e.g., viral-mediated, physical/ chemical), have been developed to achieve gene transfer. Some of the earliest methods, such as recombinant retroviruses, are still widely used, have undergone significant improvements, and have given rise to new vectors based on lentiviruses.
Reviews / Votes
Each protocol is written in a standard format, consisting of introduction, materials, methods, notes, and references. The introductions vary in length and detail. Many of them are illustrated with tables, charts, schematics, and/or photographs. The materials and methods sections are quite detailed, and there are many notes clarifying steps or outlining potential problems. Gene Therapy Protocols is recommended for university, hospital, and corporate libraries serving researchers who are working on gene therapies. - E-Streams From Reviews of the First Edition: extremely impressive a must, even for researchers who are already familiar with gene transfer techniques - Molecular Medicine Today succeeds admirably - Genetical ResearchMore details
Series
Language
English
Place of publication
NJ
United States
Target group
College/higher education
Professional and scholarly
Edition type
Revised edition
Illustrations
Illustrations
Dimensions
Height: 22.9 cm
Width: 15.2 cm
Weight
2 gr
ISBN-13
978-0-89603-723-6 (9780896037236)
DOI
10.1385/1592591418
Schweitzer Classification
Other editions
New editions
Book
04/2008
3rd Edition
Humana Press Inc.
€160.49
Shipment within 15-20 days
Joseph Ledoux
Gene Therapy Protocols
Volume 1: Production and In Vivo Applications of Gene Transfer Vectors
Book
04/2008
3rd Edition
Humana Press Inc.
€106.99
Shipment within 15-20 days
Previous edition
Christopher Robbins
Gene Therapy Protocols
Book
02/2010
Humana Press Inc.
€108.94
Article exhausted; check for reprint
Content
Poly-l-Lysine-Based Gene Delivery Systems: Synthesis, Purification, and Application
Charles P. Lollo, Mariusz G. Banaszczyk, Patricia M. Mullen, Christopher C. Coffin, Dongpei Wu, Alison T. Carlo, Donna L. Bassett, Erin K. Gouveia, and Dennis J. Carlo
Targeted Gene Transfer to Liver Using Protein-DNA Complexes
Catherine H. Wu, Cherie M. Walton, and George Y. Wu
Receptor-Directed Molecular Conjugates for Gene Transfer
Assem G. Ziady and Pamela B. Davis
Gene Transfer into Muscle by Electroporation in Vivo
Jun-ichi Miyazaki and Hiroyuki Aihara
Viral Liposomes: Preparation and Use
Yasufumi Kaneda
LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery
Yadi Tan, Mark Whitmore, Song Li, Peter Frederik, and Leaf Huang
Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use
Klaus Schughart and Ulla B. Rasmussen
Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts
Olga A. Igoucheva and Kyonggeun Yoon
Regulated Expression of Plasmid-Based Gene Therapies
Ronald V. Abruzzese, Fiona C. MacLaughlin, Louis C. Smith, and Jeffrey L. Nordstrom
Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery
Lina Prasmickaite, Anders Høgset, and Kristian Berg
Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun
Chia-Feng Kuo, Jeng Hwan Wang, and Ning-Sun Yang
Preparation of Pseudotyped Retroviral Vector
Hong Yu
Quantitative Measurement of the Concentration of Active Recombinant Retrovirus
Stelios T. Andreadis and Jeffrey R. Morgan
Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use
Jarmo Wahlfors and Richard A. Morgan
Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells
William P. Swaney,Enrico M. Novelli, Alfred B. Bahnson, and John A. Barranger
Genetically Modified Skin Substitutes: Preparation and Use
Karen E. Hamoen, Gulsun Erdag, Jennifer L. Cusick, Hinne A. Rakhorst, and Jeffrey R. Morgan
Bioartificial Muscles in Gene Therapy
Courtney Powell, Janet Shansky, Michael Del Tatto, and Herman H. Vandenburgh
Cytokine Gene-Modified Cell-Based Cancer Vaccines
R. Todd Reilly, Jean-Pascal H. Machiels, Leisha A. Emens, and Elizabeth M. Jaffee
HIV-Based Vectors: Preparation and Use
Antonia Follenzi and Luigi Naldini
Packaging Cell System for Lentivirus Vectors: Preparation and Use
Narasimhachar Srinivasakumar
Lentiviral Vectors: Preparation and Use
Lung-Ji Chang and Anne-Kathrin Zaiss
Simian Foamy Virus Vectors: Preparation and Use
Jeonghae Park and Ayalew Mergia
Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use
Michael A. Curran a
Charles P. Lollo, Mariusz G. Banaszczyk, Patricia M. Mullen, Christopher C. Coffin, Dongpei Wu, Alison T. Carlo, Donna L. Bassett, Erin K. Gouveia, and Dennis J. Carlo
Targeted Gene Transfer to Liver Using Protein-DNA Complexes
Catherine H. Wu, Cherie M. Walton, and George Y. Wu
Receptor-Directed Molecular Conjugates for Gene Transfer
Assem G. Ziady and Pamela B. Davis
Gene Transfer into Muscle by Electroporation in Vivo
Jun-ichi Miyazaki and Hiroyuki Aihara
Viral Liposomes: Preparation and Use
Yasufumi Kaneda
LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery
Yadi Tan, Mark Whitmore, Song Li, Peter Frederik, and Leaf Huang
Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use
Klaus Schughart and Ulla B. Rasmussen
Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts
Olga A. Igoucheva and Kyonggeun Yoon
Regulated Expression of Plasmid-Based Gene Therapies
Ronald V. Abruzzese, Fiona C. MacLaughlin, Louis C. Smith, and Jeffrey L. Nordstrom
Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery
Lina Prasmickaite, Anders Høgset, and Kristian Berg
Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun
Chia-Feng Kuo, Jeng Hwan Wang, and Ning-Sun Yang
Preparation of Pseudotyped Retroviral Vector
Hong Yu
Quantitative Measurement of the Concentration of Active Recombinant Retrovirus
Stelios T. Andreadis and Jeffrey R. Morgan
Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use
Jarmo Wahlfors and Richard A. Morgan
Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells
William P. Swaney,Enrico M. Novelli, Alfred B. Bahnson, and John A. Barranger
Genetically Modified Skin Substitutes: Preparation and Use
Karen E. Hamoen, Gulsun Erdag, Jennifer L. Cusick, Hinne A. Rakhorst, and Jeffrey R. Morgan
Bioartificial Muscles in Gene Therapy
Courtney Powell, Janet Shansky, Michael Del Tatto, and Herman H. Vandenburgh
Cytokine Gene-Modified Cell-Based Cancer Vaccines
R. Todd Reilly, Jean-Pascal H. Machiels, Leisha A. Emens, and Elizabeth M. Jaffee
HIV-Based Vectors: Preparation and Use
Antonia Follenzi and Luigi Naldini
Packaging Cell System for Lentivirus Vectors: Preparation and Use
Narasimhachar Srinivasakumar
Lentiviral Vectors: Preparation and Use
Lung-Ji Chang and Anne-Kathrin Zaiss
Simian Foamy Virus Vectors: Preparation and Use
Jeonghae Park and Ayalew Mergia
Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use
Michael A. Curran a