Microfluidic Techniques
Reviews and Protocols
Published on 9. December 2010
Book
Paperback/Softback
X, 248 pages
978-1-61737-613-9 (ISBN)
Description
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
More details
Series
Edition
Softcover reprint of hardcover 1st ed. 2006
Language
English
Place of publication
Totowa
United States
Target group
Professional and scholarly
Professional/practitioner
Product notice
Paperback (trade)
Unsewn / adhesive bound
Illustrations
X, 248 p.
Dimensions
Height: 229 mm
Width: 152 mm
Thickness: 15 mm
Weight
386 gr
ISBN-13
978-1-61737-613-9 (9781617376139)
DOI
10.1385/1592599974
Schweitzer Classification
Other editions
Additional editions
Book
11/2005
Humana Press Inc.
€106.99
Shipment within 15-20 days
Content
Microfabrication Methods.- Overview of Advances in Microfluidics and Microfabrication.- to Microfabrication Techniques.- Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds.- Fabrication of Microelectrodes Using the Lift-Off Technique.- Laser Ablation as a Fabrication Technique for Microfluidic Devices.- Reversible, Room Temperature Bonding of Glass Devices for Microfluidics.- Biological Applications.- Solid-Phase Extraction in Packed Beds on Glass Microdevices.- Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems.- Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays.- Polymerase Chain Reaction in Miniaturized Systems:.- Polymerase Chain Reaction on Microchips.- A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes.- Microfluidic Ethanol Biobatteries on a Microchip.- Microfluidic Chambers for Cell Migration and Neuroscience Research.- Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells.- Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip.- Microscale Integrated Sperm Sorter.