
Enzyme Stabilization and Immobilization
Methods and Protocols
Shelley D. Minteer(Editor)
Humana Press Inc.
Published on 23. August 2016
Book
Paperback/Softback
X, 229 pages
978-1-4939-5792-7 (ISBN)
Description
In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular BiologyT series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.
More details
Series
Edition
Softcover reprint of the original 1st ed. 2011
Language
English
Place of publication
Totowa
United States
Target group
Professional and scholarly
Illustrations
X, 229 p.
Dimensions
Height: 254 mm
Width: 178 mm
Thickness: 14 mm
Weight
460 gr
ISBN-13
978-1-4939-5792-7 (9781493957927)
DOI
10.1007/978-1-60761-895-9
Schweitzer Classification
Other editions
Additional editions

Book
09/2010
Humana Press Inc.
€106.99
Shipment within 15-20 days
Content
Introduction to the field of enzyme immobilization and stabilization.- Stabilization of enzymes through encapsulation in liposomes.- Micellar enzymology for thermal, pH, and solvent stability.- Lipase activation and stabilization in room temperature ionic liquids.- Nanoporous silica glass for the immobilization of interactive enzyme systems.- Enzyme stabilization and immobilization by sol-gel entrapment.- Nanoporous Gold for Enzyme Immobilization.- Enzyme stabilization via bio-templated silicification reactions.- The immobilization of enzyme on Eupergit supports by covalent attachment.- Micellar polymer encapsulation of enzymes.- Enzyme Stabilization via Cross-Linked Enzyme Aggregates.- Enzyme immobilization in polyelectrolyte microcapsules.- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization.- Enzyme-nanoparticle conjugates for biomedical applications.- Enzyme nanoparticle fabrication- Magnetic nanoparticle synthesis and enzyme immobilization.- Cytochrome C stabilization and immobilization in aerogels.- Kinetic Measurement for Enzyme Immobilization.