Enzyme Stabilization and Immobilization
Description
This third edition provides new and updated methods on enzyme stabilization. Chapters focus on protocols for enzyme stabilization, enzyme immobilization, and the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Enzyme Stabilization and Immobilization: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers in the field.
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Content
Introduction to the field of enzyme immobilization and stabilization.- Stabilization of enzymes through encapsulation in liposomes.- Micellar enzymology for thermal, pH, and solvent stability.- Lipase activation and stabilization in room temperature ionic liquids.- Stabilization of Enzymes via Covalent Tethering to Appropriately Tuned Random Copolymer Brushes.- Nanoporous Gold for Enzyme Immobilization.- Immobilization of nicotinamide adenine dinucleotide cofactors using aryl diazonium electrografting.- Micellar polymer encapsulation of enzymes.- Cytochrome C stabilization and immobilization in aerogels.- Enzyme Immobilization and Mediation with Osmium Redox Polymers for Anodes and Cathodes.- Ferrocene-Modified Linear Poly(ethylenimine) for Enzymatic Immobilization and Electron Mediation.- FAD-dependent Glucose Dehydrogenase Immobilization and Mediation within a Naphthoquinone Redox Polymer.- Cobaltocene polymers for low-potential enzymatic electrocatalysis.- Surface Immobilization of Metalloenzymes Using a Pyrene-Modified Hydrogel.- Layer-by-layer assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes.- Nitrobenzoic acid-functionalized nanomaterials for enzyme immobilization.- Immobilization of Enzyme Cascades with DNA Scaffolds.