DNA Methylation Protocols
Published on 1. March 2002
Book
Hardback
X, 189 pages
978-0-89603-618-5 (ISBN)
Description
DNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.
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12/2008
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Humana Press Inc.
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Content
Overview
Ken I. Mills and Bernard H. Ramsahoye
Nearest-Neighbor Analysis
Bernard H. Ramsahoye
Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography
Bernard H. Ramsahoye
Methylation Analysis by Chemical DNA Sequencing
Piroska E. Szabó, Jeffrey R. Mann, and Gerd P. Pfeifer
Methylation-Sensitive Restriction Fingerprinting
Catherine S. Davies
Restriction Landmark Genome Scanning
Joseph F. Costello, Christoph Plass, and Webster K. Cavenee
Combined Bisulfite Restriction Analysis (COBRA)
Cindy A. Eads and Peter W. Laird
Differential Methylation Hybridization Using CpG Island Arrays
Pearlly S. Yan, Susan H. Wei, and Tim Hui-Ming Huang
Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences
Minoru Toyota and Jean-Pierre J. Issa
Isolation of CpG Islands Using a Methyl-CpG Binding Column
Sally H. Cross
Purification of MeCP2-Containing Deacetylase from Xenopus laevis
Peter L. Jones, Paul A. Wade, and Alan P. Wolffe
DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method
Petra Hajkova, Osman El-Maarri, Sabine Engemann, Joachim Oswald, Alexander Olek, and Jörn Walter
Measuring DNA Demethylase Activity In Vitro
Moshe Szyf and Sanjoy K. Bhattacharya
Extracting DNA Demethylase Activity from Mammalian Cells
Moshe Szyf and Sanjoy K. Bhattacharya
Ken I. Mills and Bernard H. Ramsahoye
Nearest-Neighbor Analysis
Bernard H. Ramsahoye
Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography
Bernard H. Ramsahoye
Methylation Analysis by Chemical DNA Sequencing
Piroska E. Szabó, Jeffrey R. Mann, and Gerd P. Pfeifer
Methylation-Sensitive Restriction Fingerprinting
Catherine S. Davies
Restriction Landmark Genome Scanning
Joseph F. Costello, Christoph Plass, and Webster K. Cavenee
Combined Bisulfite Restriction Analysis (COBRA)
Cindy A. Eads and Peter W. Laird
Differential Methylation Hybridization Using CpG Island Arrays
Pearlly S. Yan, Susan H. Wei, and Tim Hui-Ming Huang
Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences
Minoru Toyota and Jean-Pierre J. Issa
Isolation of CpG Islands Using a Methyl-CpG Binding Column
Sally H. Cross
Purification of MeCP2-Containing Deacetylase from Xenopus laevis
Peter L. Jones, Paul A. Wade, and Alan P. Wolffe
DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method
Petra Hajkova, Osman El-Maarri, Sabine Engemann, Joachim Oswald, Alexander Olek, and Jörn Walter
Measuring DNA Demethylase Activity In Vitro
Moshe Szyf and Sanjoy K. Bhattacharya
Extracting DNA Demethylase Activity from Mammalian Cells
Moshe Szyf and Sanjoy K. Bhattacharya