Fingerprinting Methods Based on Arbitrarily Primed PCR
Published on 23. August 2014
Book
Paperback/Softback
XVI, 441 pages
978-3-642-47812-3 (ISBN)
Description
PCR has developed to the most powerful tool for the study of DNA and genes. Various techniques based on PCR are described in this manual, including protocols for the analysis of genes, mutations, abnormal gene expression in tumors and gene expression during differentiation.
Reviews / Votes
"The only primer you'll ever need... great care has been taken in putting this book together, and the results are very impressive. Although the work of many authors, the contributions form a coherent and well-organized whole." Trends in Genetics"...buy this excellent book." Cellular and Molecular Biology
More details
Series
Edition
Softcover reprint of the original 1st ed. 1997
Language
English
Place of publication
Berlin
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Research
Illustrations
XVI, 441 p.
Dimensions
Height: 254 mm
Width: 203 mm
Thickness: 25 mm
Weight
979 gr
ISBN-13
978-3-642-47812-3 (9783642478123)
DOI
10.1007/978-3-642-60441-6
Schweitzer Classification
Other editions
Additional editions
Maria R. Micheli | Rodolfo Bova
Fingerprinting Methods Based on Arbitrarily Primed PCR
E-Book
11/2013
Springer
€53.49
Available for download
Maria R. Micheli | Rodolfo Bova
Fingerprinting Methods Based on Arbitrarily Primed PCR
Book
01/1997
Springer
€85.55
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Content
Overview.- I DNA Extraction from Mammals.- II Insect DNA Extraction Protocol.- III Rapid DNA Extraction from Plants.- IV Preparation of Fungal Genomic DNA for PCR and RAPD Analysis.- V Extraction of Histoplasma capsulatum DNA for PCR.- VI DNA Extraction from Bacterial Cultures.- VII Arbitrarily Primed PCR and RAPDs.- VIII Random Amplified Polymorphic DNA Assay.- IX DNA Amplification Fingerprinting.- X Fluorescent Detection and Analysis of RAPD Amplicons Using the ABI PRISM DNA Sequencers.- XI Optimization of RAPD Fingerprinting.- XII Fingerprint Tailoring.- XIII Resolving DNA Amplification Products Using Polyacrylamide Gel Electrophoresis and Silver Staining.- XIV Denaturing Gradient Gel Electrophoresis for Enhanced Detection of DNA Polymorphisms.- XV Modified Temperature Sweep Gel Electrophoresis for the Separation of Arbitrarily Amplified DNA Fragments.- XVI High Throughput Scoring of RAPD Fragments Through the Use of Dot-Blot Hybridization.- XVII Recovering Amplified DNA from Silver Stained Gels.- XVIII Cloning of RAPD Markers.- XIX Sequencing of RAPD Markers.- XX Analysis of Tumor-Specific Genetic Alterations by Arbitrarily Primed PCR.- XXI Construction of Linkage Maps with RAPD Markers.- XXII Pseudo-Testcross Mapping Strategy Using RAPD Markers.- XXIII Estimating Nucleotide Divergence with RAPD Data.- XXIV RAPD and PAUP Analysis for Microbial Screening Programs.- XXV Production of Specific Probes for Microorganisms.- Overview.- XXVI Differential Display of Expressed mRNAs.- XXVII RNA Arbitrarily Primed PCR.- XXVIII Nonradioactive Differential Display of Messenger RNA.- XXIX Fluorescent Differential Display: A Fast and Safe Way for Reliable Differential Display Analysis.- XXX Slot Blot Hybridization Screening.- XXXI How To Find and Clone the Appropriate cDNA Fragments Generated in Differential mRNA Display by Using Northern Blot for cDNA Capture.- XXXII Verification of Differential Display Results by RNase Protection.- XXXIII Direct Automated Sequencing of DDRT-PCR Fragments.- XXXIV Ligation Linked PCR and Direct Sequencing of Differential Display Products.- XXXV Differential Display PCR Fragments as Probes for cDNA Cloning.- XXXVI Targeted RNA Fingerprinting.- XXXVII Generation of Tumor-Specific DEST Catalogue.- XXXVIII Analysis of Gene Expression in the Preimplantation Mouse Embryo Using mRNA Differential Display.- XXXIX Rapid Identification of Differentially Expressed Genes in Trypanosomes.