PCR 2
A Practical Approach
Published on 1. June 1995
Book
Paperback/Softback
358 pages
978-0-19-963424-8 (ISBN)
Description
PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical
Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis.
Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis.
Reviews / Votes
This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire. * Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6 *More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Illustrations
3 colour halftones, halftones, line figures and tables
Dimensions
Height: 234 mm
Width: 156 mm
Thickness: 20 mm
Weight
533 gr
ISBN-13
978-0-19-963424-8 (9780199634248)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
1. Optimizing PCR ; 2. Use of specialty phosphoramidites in PCR ; 3. Chemical methods for 5' non-isotopic labelling of PCR probes and primers ; 4. Solid-phase PCR ; 5. Solid-phase sequencing of PCR products ; 6. cDNA cloning by RT - PCR ; 7. Quantification of DNA and RNA by PCR ; 8. PCR MIMICS: competitive DNA fragments for use in quantitative PCR ; 9. In vitro expression of proteins from PCR products ; 10. PCR-based approaches to human genome mapping ; 11. Fingerprinting of DNA and RNA using arbitrarily primed PCR ; 12. Mutational analysis ; 13. Mutational analysis: new mutations ; 14. Linear amplification for the in virto study of ligand/DNA interactions ; 15. Ligand-mediated PCR