Directed Mutagenesis
Published on 1. June 1991
Book
Hardback
277 pages
978-0-19-963141-4 (ISBN)
Description
Directed mutagenesis has emerged as an essential tool in both genetic and protein engineering by facilitating both molecular analysis and the modification of the structural and functional properties of cloned DNA sequences. Techniques have been refined to the point where virtually any desired change or series of changes in the nucleotide sequence may be rapidly introduced. Several methods also exist for the introduction of random rather than targeted mutations, thus allowing the identification of structurally or functionally important regions that might not otherwise be detected. This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences. It provides up-to-date protocols and practical advice that should be of use to the beginner who wishes to be guided through the available approaches, and to the experienced researcher interested in exploring alternative procedures.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
numerous halftones, line drawings and tables, bibliography
ISBN-13
978-0-19-963141-4 (9780199631414)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
Mutagenesis facilitated by the removal or introduction of unique restriction sites, Paul Carter; efficient site-directed in vitro mutagenesis using uracil-containing DNA and phagemid vectors, P.D.Yuckenberg, F.Witney, J.Geisselsoder and J.McClary; phosphorothioate-based site-directed mutagenesis for single-stranded vectors, J.Sayers and F.Eckstein; site-specific mutagenesis using double-stranded plasmid DNA, S.Inouye and M.Inouye; phosphorothioate-based double-stranded plasmid mutagenesis, D.M.Olsen and F.Eckstein; linker scanning mutagenesis, B.Luckow and G.Shutz; random chemical mutagenesis and the non-selective isolation of mutated DNA sequences in vitro, C.Walton, R.K.Booth and P.G.Stockley; an enzymatic method for the complete mutagenesis of genes, J.K.C.Knowles and P.Lehtovaara; spiked oligonucleotide mutagenesis, S.C.Blacklow and J.R.Knowles; cassette mutagenesis, J.H.Richards; recombination and mutagenesis of DNA sequences using PCR, R.M.Horton and L.R.Pease.