Directed Mutagenesis: A Practical Approach
Published on 27. June 1991
Book
Paperback/Softback
278 pages
978-0-19-963140-7 (ISBN)
Description
Directed mutagenesis has emerged as an essential tool in both genetic and protein engineering by facilitating both molecular analysis and the modification of the structural and functional properties of cloned DNA sequences. Techniques have been refined to the point where virtually any desired change or series of changes in the nucleotide sequence may be rapidly introduced. Several methods also exist for the introduction of random rather than targeted mutations, thus allowing the identification of structurally or functionally important regions that might not otherwise be detected. This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences. It provides up-to-date protocols and practical advice that should be of use to the beginner who wishes to be guided through the available approaches, and to the experienced researcher interested in exploring alternative procedures.
Reviews / Votes
'The text is presented in such a way as to be suitable for both beginner and expert, especially those interested in exploring alternative procedures.'ASLIB Booklist, Vol.56, No.10, October 1991 'This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences. It provides up-to-date protocols and practical advice that will prove valuable to the beginner who wishes to be guided through the available approaches, and to the experienced researcher interested in exploring alternative procedures.'
Biochimica Clinica, 1992, Vol. 16, No. 6 'The latest in the Practical Approach series ... is all that you would expect from its long line of predecessors. An illustrious list of contributors includes many leaders in their respective fields as well as the pioneers of the techniques they describe. This alone lends authority to and instils confidence in each method.'
Daniel C. Emery, University of Bristol, BioEssays, Vol. 15, No. 4 - April 1993
More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
numerous halftones, line drawings and tables
Dimensions
Height: 232 mm
Width: 155 mm
Thickness: 15 mm
Weight
422 gr
ISBN-13
978-0-19-963140-7 (9780199631407)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Person
Content
Paul Carter: Mutagenesis Facilitated by the Removal or Introduction of Unique Restriction Sites; P. D. Yuckenberg, F. Witney, J. Geisselsoder, & J. McClary: Efficient Site-directed In Vitro Mutagenesis using Uracil-containing DNA and Phagemid Vectors; J. Sayers & F. Eckstein: Phosphorothioate-based Site-directed Mutagenesis for Single-stranded Vectors; S Inouye & M. Inouye: Site-specific Mutagenesis using Double-stranded Plasmid DNA; D.M. Olsen & F.
Eckstein: Phosphorothioate-based Double-stranded Plasmid Mutagenesis; B. Luckow & G. Shutz: Linker Scanning Mutagenesis; C. Walton, R.K. Booth, & P.G. Stockley: Random Chemical Mutagenesis and the Non-selective Isolation of Mutated DNA Sequences In Vitro; J.K.C. Knowles & P. Lehtovaara: An Enzymatic Method for the
Complete Mutagenesis of Genes; S.C. Blacklow & J.R. Knowles: Spiked Oligonucleotide Mutagenesis; J.H. Richards: Cassette Mutagenesis; R.M. Horton & L.R. Pease: Recombination and Mutagenesis of DNA Sequences using PCR
Eckstein: Phosphorothioate-based Double-stranded Plasmid Mutagenesis; B. Luckow & G. Shutz: Linker Scanning Mutagenesis; C. Walton, R.K. Booth, & P.G. Stockley: Random Chemical Mutagenesis and the Non-selective Isolation of Mutated DNA Sequences In Vitro; J.K.C. Knowles & P. Lehtovaara: An Enzymatic Method for the
Complete Mutagenesis of Genes; S.C. Blacklow & J.R. Knowles: Spiked Oligonucleotide Mutagenesis; J.H. Richards: Cassette Mutagenesis; R.M. Horton & L.R. Pease: Recombination and Mutagenesis of DNA Sequences using PCR