Antibody Engineering
A Practical Approach
Published on 27. June 1996
Book
Paperback/Softback
348 pages
978-0-19-963592-4 (ISBN)
Description
Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.
Reviews / Votes
This book is very useful for practising antibody engineers, providing extra information that enables them to adopt new techniques or expand into new areas. ... this is a useful and interesting resource for practising antibody engineers and I will make sure my students have access to it. * SGM Quarterly, August 1997 *More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Illustrations
halftones, line figures, tables
Dimensions
Height: 234 mm
Width: 156 mm
Thickness: 19 mm
Weight
535 gr
ISBN-13
978-0-19-963592-4 (9780199635924)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
1. Construction and use of antibody gene repertoires ; 2. Affinity maturation of antibodies using phage display ; 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs ; 4. Measuring antibody affinities in solution ; 5. Measuring antibody affinities using Bio-sensors ; 6. Analysis of human antibody sequences ; 7. Rodent to human antibodies by CDR grafting ; 8. Rodent to human antibodies by phage display ; 9. Choosing and manipulating effector functions ; 10. Secretion and purification of antibody fragments expressed in bacteria ; 11. Production of single chain Fv monomers and multimers ; 12. Expression of immunoglobulin genes in mammalian cells ; 13. In vitro linkage of antibody fragments into dimeric and multimeric forms