Animal Cell Culture: Principles and Practice
Published on 2. February 2024
Book
Paperback/Softback
XVI, 327 pages
978-3-031-19487-0 (ISBN)
Description
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
More details
Series
Edition
2023 ed.
Language
English
Place of publication
Cham
Switzerland
Publishing group
Springer International Publishing
Target group
Professional and scholarly
Illustrations
6 s/w Abbildungen, 85 farbige Abbildungen
XVI, 327 p. 91 illus., 85 illus. in color.
Dimensions
Height: 235 mm
Width: 155 mm
Thickness: 19 mm
Weight
522 gr
ISBN-13
978-3-031-19487-0 (9783031194870)
DOI
10.1007/978-3-031-19485-6
Schweitzer Classification
Other editions
Additional editions
Shalini Mani | Manisha Singh | Anil Kumar
Animal Cell Culture: Principles and Practice
Book
02/2023
Springer
€149.79
Shipment within 15-20 days
Persons
Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India.
Content
1. Overview to animal cell culture.- 2. Cell culture laboratory.- 3. Good lab practices and bio-safety measures.- 4. Managing sterility in the lab.- 5. Media and buffer preparation for cell culture.- 6. Properties of cultured cells and selection of culture media.- 7. Selection and maintenance of cultured cells.- 8. Inoculation and passaging of adherent cells.- 9. Counting of cells.- 10. Cryopreservation of cell lines.- 11. Resuscitation of frozen cell line.- 12. Isolation and culturing of cells from different tissues.- 13. Differentiation, transformation and immortalization of cells.- 14. Co-culture techniques.- 15. 3d cell culture techniques.- 16. Stem cell culture techniques.- 17. Identification and removal of biological contamination in the media and cell suspensions.- 18. Analysis of cell growth kinetics in suspension and adherent types cell line.- 19. Applications of animal cell culture based assays.- 20. Ethical issues in animal cell culture.- 21. Common troubleshooting methods in cell culture techniques.