Virology Methods Manual

Part 1 Classical techniques: Cell culture, V.C. George et al; virus isolation and quantitation, J.C. Hierholzer et al; preparation of antigens, J.R. Hierholzer et al; virus purification, R.A. Killington et al; electron microscopy, I.L. Chrystie; traditional and serological tests, M. Chernesky; immunoassays, M. Chernesky and J. Mahoney; cell mediated immunity, A. Mawle. Part 2 Techniques in molecular virology: RNA - transcription, transfection and quantitation, J.E. Novak et al; DNA viruses - DNA extraction, purification and characterization, D.J. McCance; virus mutants, K.N. Leppard and C.R. Pringle; polypeptides, D.R. Harper and B.F. Coles; the assay of viral enzyme, M. McCrae. Part 3 Medical virology: initial in vitro screening of drug candidates for their potential antiviral activities, A.L. Jabri et al; detection of virus and viral antigens, S. Specter and D. Jeffries; detection of viral nucleic acids in clinical material, J. Mahoney; serological methods, S. Specter and D. Jeffries; disinfection, S. Specter and D. Jeffries.