Enzyme Engineering

Preparation of Soil Metagenome Libraries and Screening for Gene Specific Amplicons.- Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics.- Synthetic DNA Libraries for Protein Engineering toward Process Improvement in Drug Synthesis.- In Silico Prediction Methods for Site-Saturation Mutagenesis.- Recombination of Compatible Substitutions by 2GenReP and InSiReP.- Using the Evolutionary History of Proteins to Engineer Insertion-Deletion Mutants from Robust, Ancestral Templates Using Graphical Representation of Ancestral Sequence Predictions (GRASP).- Resurrecting Enzymes by Ancestral Sequence Reconstruction.- Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactors.- Rational-Design Engineering to Improve Enzyme Thermostability.- Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents.- In Silico Engineering of Enzyme Access Tunnels.- Quantum-Mechanical/Molecular-Mechanical (QM/MM) Simulations for Understanding Enzyme Dynamics.- Computational Enzyme Design at Zymvol.- Advanced Enzyme Immobilization Technologies: An Eco-Friendly Support, a Polymer-Stabilizing Immobilization Strategy, and an Improved Cofactor Co-Immobilization Technique.- Chemical Reaction Engineering to Understand Applied Kinetics in Free Enzyme Homogeneous Reactors.- Enzymatic Oxidative Cascade for Oxofunctionalization of Fatty Acids in One-Pot.- CRISPR-Cas9 Editing of the Synthesis of Biodegradable Polyesters Polyhydroxyalkanaotes (PHA) in Pseudomonas putida KT2440.