Identification and Expression of G-protein Coupled Receptors
Published on 19. March 1998
Book
Hardback
236 pages
978-0-471-18310-5 (ISBN)
Description
Receptors are sites and system within the brain and central nervous system that act as links in the information processing chains. G-protein receptors (GPCRs), the biggest single class of receptors in biology, are the primary effectors of the senses of smell and sight. A large number of current drugs and addictive agents can block and/or mimic these GPCRs. This manual reflects the increased focus on approaches to ligand identification by emphasizing expression, rather than primary cloning strategies. Each contributor provides detailed protocols for executing various strategies and offers judgments as to what approaches are, and are not useful.
More details
Series
Language
English
Place of publication
New York
United States
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Illustrations
Illustrations
Dimensions
Height: 260 mm
Width: 185 mm
Weight
652 gr
ISBN-13
978-0-471-18310-5 (9780471183105)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Kevin R. Lynch
Identification and Expression of G Protein-Coupled Receptors
Book
05/1999
Wiley
€212.50
Shipment within 10-20 days
Content
Cloning of G Protein-coupled receptor genes - the use of homology screening and the polymerase chain reaction; practical approaches for cloning G Protein-coupled receptors using mammalian cell expression; GPRC informatics and the orphan problem; the use of Xenopus laevis oococytes for the study of G Protein-coupled receptors; stable expression of G Protein-coupled receptors in mammalian cells; heterologous expression in primary cell cultures; expression of G Protein-coupled receptors in escherichia coli; large scale expression/purification of G Protein-coupled receptors - mammalian cells; high-level expression of G Protein-coupled receptors in the baculovirus/Sf9 cell expression system; expression of G Protein-coupled receptors in drosophila schneider 2; heterologous G Protein-coupled receptors expressed in saccharomyces cerevisiae - methods for genetic analysis and ligand identification.