Protocols for Gene Transfer in Neuroscience
Towards Gene Therapy of Neurological Disorders
Published on 26. April 1996
Book
Paperback/Softback
XXVI, 418 pages
978-0-471-95766-9 (ISBN)
Description
This is a study of the latest techniques used by gene delivery laboratories. The methodology under discussion includes physical transfection techniques, various viral vectors, and retrovirally-transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.
More details
Language
English
Place of publication
Chichester
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Illustrations
index
Dimensions
Height: 24.5 cm
Width: 21 cm
Weight
1030 gr
ISBN-13
978-0-471-95766-9 (9780471957669)
Schweitzer Classification
Content
PROTOCOLS FOR GENE DELIVERY TO CULTURED CELLS USING PHYSICAL METHODS: Gene Therapy into Post-Mitotic Neurons in Culture by Microinjection; PROTOCOLS FOR GENE DELIVERY USING RNA VIRUSES: Semliki Forest Virus Vectors for Gene Transfer; PROTOCOLS FOR GENE DELIVERY USING ADENOVIRUSES AND ADENO-ASSOCIATED VIRUSES: Adeno-Associated Virus Vectors for Gene Transfer; PROTOCOLS FOR GENE DELIVERY USING HERPES SIMPLEX VIRUS 1 VECTORS: Transcriptional Regulation of Herpes Simplex Virus Gene Expression: Implications for our Understanding of Latency; PROTOCOLS FOR GENE DELIVERY USING CELLS: Murine Mucopolysaccharides Type VII: A Model System for Somatic Gene Therapy of the Central Nervous System; PROTOCOLS FOR IN VIVO APPLICATION OF GENE TRANSFER METHODS: Replication-Competent Viral Therapy of Tumours in the Central Nervous System; PROSPECTS AND SHORTCOMINGS OF NEUROLOGICAL GENE THERAPY.