Laboratory Methods in Enzymology: RNA: Volume 530
Published on 1. November 2013
Book
Hardback
472 pages
978-0-12-420037-1 (ISBN)
Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Dimensions
Height: 229 mm
Width: 152 mm
Weight
870 gr
ISBN-13
978-0-12-420037-1 (9780124200371)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Jon Lorsch
Laboratory Methods in Enzymology: RNA
E-Book
09/2013
Academic Press
€155.00
Available for download
Person
Content
Computational Prediction of RNA Secondary Structure
Reverse-transcription PCR (RT-PCR)
Northern blotting
Explanatory Chapter: Nuclease Protection Assays
In Vitro Transcription from Plasmid or PCR-amplified DNA
Measurement of In Vivo RNA Synthesis Rates
Method for measuring mRNA decay rate in Saccharomyces cerevisiae
Analysis of polysomes from bacteria
Polysome Profile Analysis - Yeast
Polysome analysis of mammalian cells
Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae
Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates
Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'; ends, and poly(A) tails of RNA
RNA Radiolabeling
Fluorescently Labeling Synthetic RNAs
Analysis of RNA by analytical polyacrylamide gel electrophoresis
RNA purification by preparative polyacrylamide gel electrophoresis
Explanatory Chapter: Nucleic Acid Concentration Determination
RNA purification - precipitation methods
Reverse Transcriptase Dideoxy Sequencing of RNA
RNA Structure Experimental Analysis - Chemical Modification
Structural Analysis of RNA Backbone Using In-Line Probing
Reverse-transcription PCR (RT-PCR)
Northern blotting
Explanatory Chapter: Nuclease Protection Assays
In Vitro Transcription from Plasmid or PCR-amplified DNA
Measurement of In Vivo RNA Synthesis Rates
Method for measuring mRNA decay rate in Saccharomyces cerevisiae
Analysis of polysomes from bacteria
Polysome Profile Analysis - Yeast
Polysome analysis of mammalian cells
Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae
Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates
Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'; ends, and poly(A) tails of RNA
RNA Radiolabeling
Fluorescently Labeling Synthetic RNAs
Analysis of RNA by analytical polyacrylamide gel electrophoresis
RNA purification by preparative polyacrylamide gel electrophoresis
Explanatory Chapter: Nucleic Acid Concentration Determination
RNA purification - precipitation methods
Reverse Transcriptase Dideoxy Sequencing of RNA
RNA Structure Experimental Analysis - Chemical Modification
Structural Analysis of RNA Backbone Using In-Line Probing