
Laboratory Methods in Enzymology: Protein Part D: Volume 559
Laboratory Methods in Enzymology
Jon Lorsch(Editor)
Academic Press
Published on 9. June 2015
Book
Hardback
160 pages
978-0-12-800279-7 (ISBN)
Description
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.
In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts.
In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
College/higher education
Dimensions
Height: 229 mm
Width: 152 mm
Weight
390 gr
ISBN-13
978-0-12-800279-7 (9780128002797)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions

Laboratory Methods in Enzymology: Protein Part D
Laboratory Methods in Enzymology
E-Book
06/2015
Academic Press
€142.00
Available for download
Person
Volume editor
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Content
1. Purification of His-tagged ProteinsAnne Spriestersbach, Jan Kubicek, Frank Schaefer, Helena Block and Barbara Maertens2. Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) TagKrisna C. Duong-Ly and Sandra B. Gabelli3. Immunoaffinity Purification of ProteinsJennifer M. Kavran and Daniel J. Leahy4. Affinity Purification of Protein Complexes Using TAP TagsErica Gerace and Danesh Moazed5. Strep-tagged Protein PurificationBarbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schaefer6. Proteolytic Affinity Tag CleavageHelena Block, Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schaefer7. Affinity Pull-down of Proteins using anti-FLAG M2 Agarose BeadsErica Gerace and Danesh Moazed8. Protein Affinity Purification using Intein/Chitin Binding Protein TagsSarah F. Mitchell and Jon R. Lorsch9. Purification of GST-tagged ProteinsFrank Schaefer, Nicole Seip, Barbara Maertens, Helena Block and Jan Kubicek