
Laboratory Methods in Enzymology: Protein Part C: Volume 541
Jon Lorsch(Editor)
Academic Press
Published on 9. June 2014
Book
Hardback
296 pages
978-0-12-420119-4 (ISBN)
Description
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Dimensions
Height: 229 mm
Width: 152 mm
Weight
590 gr
ISBN-13
978-0-12-420119-4 (9780124201194)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
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Other editions
Additional editions

Laboratory Methods in Enzymology: Protein Part C
Protein Part C
E-Book
03/2014
Academic Press
€155.00
Available for download
Person
Volume editor
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Content
TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein-Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein-Nucleic Acid Interactions - Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates