Laboratory Methods in Enzymology: Protein Part B: Volume 539
Protein Part B
Published on 29. April 2014
Book
Hardback
240 pages
978-0-12-420120-0 (ISBN)
Description
Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Product notice
Laminated cover
Dimensions
Height: 229 mm
Width: 152 mm
Thickness: 14 mm
Weight
492 gr
ISBN-13
978-0-12-420120-0 (9780124201200)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
E-Book
02/2014
Academic Press
€155.00
Available for download
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Content
Small-scale expression of proteins in E. coli
Protein expression-Yeast
Recombinant protein expression in baculovirus-infected insect cells
Single cell cloning of a stable mammalian cell line
In vitro synthesis of proteins in bacterial extracts
Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
Yeast two-hybrid screen
UV cross-linking of interacting RNA and protein in cultured cells
Analysis of RNA-protein interactions by cell mixing
General protein-protein cross-linking
Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
Dissecting a known RNA-protein interaction using a yeast three-hybrid system
Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system
Protein expression-Yeast
Recombinant protein expression in baculovirus-infected insect cells
Single cell cloning of a stable mammalian cell line
In vitro synthesis of proteins in bacterial extracts
Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
Yeast two-hybrid screen
UV cross-linking of interacting RNA and protein in cultured cells
Analysis of RNA-protein interactions by cell mixing
General protein-protein cross-linking
Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
Dissecting a known RNA-protein interaction using a yeast three-hybrid system
Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system