Laboratory Methods in Enzymology: Protein Part A: Volume 536
Protein Part a
Published on 25. March 2014
Book
Hardback
200 pages
978-0-12-420070-8 (ISBN)
Description
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.
This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including:
Pulse-chase analysis to measure protein degradation
Labeling a protein with fluorophores using NHS ester derivitization
Immunoaffinity purification of proteins
Proteolytic affinity tag cleavage
Purification of GST-tagged proteins
This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including:
Pulse-chase analysis to measure protein degradation
Labeling a protein with fluorophores using NHS ester derivitization
Immunoaffinity purification of proteins
Proteolytic affinity tag cleavage
Purification of GST-tagged proteins
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Product notice
Laminated cover
Dimensions
Height: 229 mm
Width: 152 mm
Thickness: 13 mm
Weight
437 gr
ISBN-13
978-0-12-420070-8 (9780124200708)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
E-Book
01/2014
Academic Press
€155.00
Available for download
Person
Content
Practical Steady-state Enzyme Kinetics
Quantification of Protein Concentration using UV absorbance and Coomassie Dyes
Preparation of protein samples for mass spectrometry and N-terminal sequencing
Lysis of mammalian and Sf9 cells
In Vivo [35 S]-Methionine Incorporation
Pulse-chase analysis to measure protein degradation
Labeling of a Protein with Fluorophores using Maleimide Derivitization
Labeling a protein with fluorophores using NHS ester derivitization
Protein Derivitization-Expressed Protein Ligation
Protein Biotinylation
Purification of His-tagged proteins
Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
Immunoaffinity purification of proteins
Affinity Purification of Protein Complexes Using TAP Tags
Strep-tagged protein purification
Proteolytic affinity tag cleavage
Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
Protein Affinity Purification using Intein/Chitin Binding Protein Tags
Purification of GST-tagged proteins
Quantification of Protein Concentration using UV absorbance and Coomassie Dyes
Preparation of protein samples for mass spectrometry and N-terminal sequencing
Lysis of mammalian and Sf9 cells
In Vivo [35 S]-Methionine Incorporation
Pulse-chase analysis to measure protein degradation
Labeling of a Protein with Fluorophores using Maleimide Derivitization
Labeling a protein with fluorophores using NHS ester derivitization
Protein Derivitization-Expressed Protein Ligation
Protein Biotinylation
Purification of His-tagged proteins
Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
Immunoaffinity purification of proteins
Affinity Purification of Protein Complexes Using TAP Tags
Strep-tagged protein purification
Proteolytic affinity tag cleavage
Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
Protein Affinity Purification using Intein/Chitin Binding Protein Tags
Purification of GST-tagged proteins